Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 84489..85014 Replicon plasmid pAR-0428-1
Accession NZ_CP044146
Organism Escherichia coli O157 strain AR-0428

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag F4V40_RS28980 Protein ID WP_001159868.1
Coordinates 84489..84794 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag F4V40_RS28985 Protein ID WP_000813634.1
Coordinates 84796..85014 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V40_RS28955 79692..80858 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
F4V40_RS28960 81446..81784 - 339 WP_071525396.1 RepB family plasmid replication initiator protein -
F4V40_RS28965 81746..82959 + 1214 WP_162829202.1 IS3 family transposase -
F4V40_RS28975 83682..84488 - 807 WP_000016989.1 site-specific integrase -
F4V40_RS28980 84489..84794 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
F4V40_RS28985 84796..85014 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F4V40_RS28990 85473..86156 + 684 WP_010891291.1 DNA-binding protein -
F4V40_RS28995 86153..86773 + 621 WP_001248529.1 hypothetical protein -
F4V40_RS29000 86770..86970 + 201 WP_000708307.1 hypothetical protein -
F4V40_RS29010 87378..88355 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
F4V40_RS29015 88640..89380 - 741 WP_001066920.1 tyrosine-type recombinase/integrase -
F4V40_RS29020 89501..89731 - 231 WP_010891288.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyA / hlyC / exeG / exeE / stcE / espP / toxB / hlyD / hlyD / hlyB 1..92722 92722


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T140271 WP_001159868.1 NZ_CP044146:c84794-84489 [Escherichia coli O157]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T140271 NZ_CP059068:2245406-2245807 [Rhodoferax sp. AJA081-3]
ATGTTGAAGTATCTTTTGGATACCAACATCGTCATCTACGTCATCAAGCGCAAGCCCCTGAATGCGCTGCAGACCTTCAA
CCTGCATGCCGGGCATATGGCCATATCGTCCATCACGCTTGCCGAACTGCTGCATGGCGCCGAGAAGAGCACCGCTCCCG
CGCAGACACTGGCTGTGGTGGAAGATTTTTGCAGCCGCCTGGAAATCTTGACCTATGGCCCCAAGGCGGCGCAGCACTAC
GGGAGCATCCGCGCCAGTCTGGAAAAAATTGGCAAACCTATCGGTGTTAATGACATCCACATTGCAGCCCACGCGCGCAG
CGAAGGCCTGACCCTGGTGACCAACAACCTGCGTGAATTCGAACGCGTGCCTGCCCTGCAATTCGAAAACTGGGTAGCCT
GA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT140271 WP_000813634.1 NZ_CP044146:c85014-84796 [Escherichia coli O157]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT140271 NZ_CP059068:2245173-2245406 [Rhodoferax sp. AJA081-3]
ATGTCCAACAGCACCGTTTTCACCAACAACCGAAGCCAAGCCATACGCCTACCGGCCGACATGCGCCTGCCCGCTTCTGT
GAAGCGCGTCACGGTCCGGGCGCGGGGCATGGAACGCATCATCACGCCCATCGAAAACACGTGGGACAGTTTCTTTTTAG
ACTCTCCCAACGTGCCGGAAGACTTTCTGCCCACACGCGCCAGCCAGGAGCAGCCGGACCGAGAGAGTTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References