Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 38819..39378 Replicon plasmid pAR-0428-1
Accession NZ_CP044146
Organism Escherichia coli O157 strain AR-0428

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag F4V40_RS28670 Protein ID WP_000421248.1
Coordinates 38819..39094 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag F4V40_RS28675 Protein ID WP_001178089.1
Coordinates 39094..39378 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V40_RS28635 34172..34561 - 390 WP_001172748.1 cytochrome b562 family protein -
F4V40_RS28640 34605..36815 - 2211 WP_000592771.1 catalase/peroxidase KatP -
F4V40_RS28645 36992..37177 + 186 WP_001302179.1 hypothetical protein -
F4V40_RS28650 37177..37593 + 417 Protein_30 IS91 family transposase -
F4V40_RS28655 37593..38159 + 567 Protein_31 IS3-like element IS600 family transposase -
F4V40_RS28660 38203..38418 + 216 Protein_32 transposase -
F4V40_RS28665 38518..38724 - 207 WP_001105064.1 hypothetical protein -
F4V40_RS28670 38819..39094 - 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F4V40_RS28675 39094..39378 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
F4V40_RS28690 40290..41147 - 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
F4V40_RS28695 41140..41214 - 75 WP_001370046.1 RepA leader peptide Tap -
F4V40_RS28705 41450..41704 - 255 WP_000083831.1 replication regulatory protein RepA -
F4V40_RS28710 41944..42282 - 339 WP_000766796.1 hypothetical protein -
F4V40_RS28715 42320..42529 - 210 WP_001302200.1 hemolysin expression modulator Hha -
F4V40_RS28720 42575..43036 - 462 WP_001233853.1 thermonuclease family protein -
F4V40_RS28725 43281..43493 - 213 WP_001302189.1 ANR family transcriptional regulator -
F4V40_RS28730 43626..44186 - 561 WP_000840472.1 fertility inhibition protein FinO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyA / hlyC / exeG / exeE / stcE / espP / toxB / hlyD / hlyD / hlyB 1..92722 92722


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T140270 WP_000421248.1 NZ_CP044146:c39094-38819 [Escherichia coli O157]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T140270 NZ_CP059068:1493241-1493624 [Rhodoferax sp. AJA081-3]
ATGAGTGTGTTGATCGACACCTCGGTGTGGTGTGACCATTTCCGCCGTCGCAATGGCGTGCTGGCGGAATTGATTCAACT
CGATCAGGCCCTCACCCACCCCCTGGTTGTCGGTGAACTGGCCTGCGGCACCCCGCCAGAGCCTCGCGTAAGGACCTTGG
GCGACATCGAATTGTTGCAAGCCGCCAACCAGGCCAGCGTTGACGAAGTCAGAGCGTTTATTGAGCGTGAAAAGTTGTAC
GGACTGGGATGCGGCTGGGTGGACTTTTCGTTGCTGGCCTCAACCCTGATTACACCCGGCGCACGGCTGTGGACGCTGGA
CAAACGCCTTGCTGAGCTTGCTGTGCGGTTTGGGCGCAGCTTCATCACGCCATCGGTCCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT140270 WP_001178089.1 NZ_CP044146:c39378-39094 [Escherichia coli O157]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT140270 NZ_CP059068:1493038-1493244 [Rhodoferax sp. AJA081-3]
ATGAGAACCACCGTCACCATTGAAGACGCCCTGTACGAAAAGGCTTTGGAAGTCGCCGACCCCACGATGGAGAAGTCGGA
AATCTTTCGTGAGGCCATGAAGACTTTCGTGCGGGTGCAGGCCGCTAAACGCCTGGCCGCTCTTGGCGGAACCTTGCCGA
ACATGCAGGACATTCCCCGCCGTGGCTCGGATATCACCACCCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References