Detailed information of TA system TA140268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5321256..5321806
• Replicon: chromosome
• Accession: NZ_CP044145
• Organism: Escherichia coli O157 strain AR-0428

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: F4V40_RS27410
• Protein ID: WP_001160975.1
• Coordinates: 5321492..5321806 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: F4V40_RS27405
• Protein ID: WP_000125566.1
• Coordinates: 5321256..5321489 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V40_RS27380	5316330..5316617	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
F4V40_RS27385	5316675..5318006	+	1332	WP_001183177.1	MFS transporter	-
F4V40_RS27390	5318114..5318644	+	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
F4V40_RS27395	5318637..5320499	+	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
F4V40_RS27400	5320691..5321170	+	480	WP_000624372.1	type 3 dihydrofolate reductase	-
F4V40_RS27405	5321256..5321489	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
F4V40_RS27410	5321492..5321806	+	315	WP_001160975.1	CcdB family protein	Toxin
F4V40_RS27415	5321803..5322651	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
F4V40_RS27420	5322658..5323035	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
F4V40_RS27425	5323038..5323859	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
F4V40_RS27430	5323856..5324845	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
F4V40_RS27435	5324845..5326131	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T140268 WP_001160975.1 NZ_CP044145:5321492-5321806 [Escherichia coli O157]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T140268 NZ_CP059068:c1019072-1018674 [Rhodoferax sp. AJA081-3]
ATGACCTATCTCCTGGATACCAATATTTGCATCTATGTGATCAACGAGCAACCTGCGCACGTTCTGCAGCGCTTGATTCA
GGTCGGTCGCGAGTCACTGGTGATTTCCACCGTCACGGTGGCGGAGTTGGCCTTTGGCATTGAAAAAAGCAAACGCCCCG
GGGCCCGCGCCAAGCTGGAGAATTTTCTGTCGAAGTTCCCCATACAGGATTGGGGTCAGGATGCCGCCTGGGTGTACGGG
CGGGTACGCAAATCGCTGGAGGCCAAAGGGCAACGCATCGGCGAGCGGGACTTGCTGCTGGCTTGCCAAGCCTTGGCACT
CGACGCCACTATGGTTACCAACAACACCCGCGAGTTCGAGCGTGTTGAGGGCTTGAAGCTGGAGAACTGGGTTGTCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT140268 WP_000125566.1 NZ_CP044145:5321256-5321489 [Escherichia coli O157]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT140268 NZ_CP059068:c1019359-1019069 [Rhodoferax sp. AJA081-3]
ATGACCGCCACAACCCGAACCACCACGGATTCTGCGATGCCGGCGCCAAGCGTCGCCAAGGTATTCACCACTGGCCGTAG
CCAGGCCGTACGCCTACCCAAGGCGTTTCGCTTCAACACAGCGGAAGTGACCATTGAGCGGCAAGGGGATGCCATCATTC
TGCGACCGAAACCAGACCGCGAAACCTGGGCGCAACAGGTGTTGGCCGCAGTGGCCGCGTTTGAGCCCGACTTCAAGCTG
GAGCGCAGCGACGAGTGGCCCCAACCCGATCGGGAGCCCTTGTTGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9