Detailed information of TA system TA140267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5071926..5072521
• Replicon: chromosome
• Accession: NZ_CP044145
• Organism: Escherichia coli O157 strain AR-0428

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: F4V40_RS26355
• Protein ID: WP_000239579.1
• Coordinates: 5072171..5072521 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: F4V40_RS26350
• Protein ID: WP_001223210.1
• Coordinates: 5071926..5072177 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V40_RS26340	5067591..5071370	+	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-
F4V40_RS26345	5071373..5071714	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
F4V40_RS26350	5071926..5072177	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
F4V40_RS26355	5072171..5072521	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
F4V40_RS26360	5072601..5073131	-	531	WP_000055075.1	inorganic diphosphatase	-
F4V40_RS26365	5073441..5074397	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
F4V40_RS26370	5074537..5076039	+	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
F4V40_RS26375	5076053..5077075	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T140267 WP_000239579.1 NZ_CP044145:5072171-5072521 [Escherichia coli O157]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T140267 NZ_CP059068:c832674-832342 [Rhodoferax sp. AJA081-3]
ATGAAGTGGGAGATCAAATATCACGATGAGAAGTTGCAGGCTGATGTGCTGGCATTGCCGCCGGGAATTTTGGCCAGATA
CTTTCATTGCACCGATCGTATGGTTGAGTTTGGTCCAGATTTAGGAATGCCTCACACGCGGGCAATGAGCATGGGGCTTT
TCGAGTTGCGTTTGAAATCCCGTGAGGGTATTGGGCGGGTGTTTTATTGCACTTTGGTGGATCGCAAGATCGTCATGCTC
CACCAGTTTGTAAAGAAGACCGATAAGACACCTCCTAAAGAATTGGCACTTGCGCGCAAGCGAATGAAGGAGTGGAAAAA
TGTTGACACATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT140267 WP_001223210.1 NZ_CP044145:5071926-5072177 [Escherichia coli O157]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT140267 NZ_CP059068:c832355-832008 [Rhodoferax sp. AJA081-3]
ATGTTGACACATAAACAACTTCGCACCAAGGCATTGGCTACTGCTGAGGTAAAGGCGGAATACGAAAAACTGGCTGACGA
GTTTTCGCTTTTGGACGAATTCTTGAAGGCTCGGGCGGCGCAAGGTCTGACCCAAGCGCAGGTGGCAGAAAAGATCGGTA
CCACGCAGTCTGCAGTTGCGCGTATGGAATCGGGTAGTGGCAAGCATTCACCATCTCTGGCAACGCTGACCAAGTATGCA
GATGCCTTGGGGTGCAAGTTGGAGGTTCGTTTGGTCCGCAGGCCTCGGTCAACGAAGAATGCAACTGGTCGTCCAAGCCC
GACGACGCCCAAACGTGTTGCTGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8