Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4166213..4166844 Replicon chromosome
Accession NZ_CP044145
Organism Escherichia coli O157 strain AR-0428

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag F4V40_RS22090 Protein ID WP_001259386.1
Coordinates 4166569..4166844 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag F4V40_RS22085 Protein ID WP_000593555.1
Coordinates 4166213..4166572 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V40_RS22055 4161325..4161798 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
F4V40_RS22060 4161795..4162076 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
F4V40_RS22065 4162153..4163511 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
F4V40_RS22070 4163504..4165012 + 1509 WP_001301773.1 carbohydrate kinase -
F4V40_RS22075 4165002..4165271 + 270 WP_000933060.1 HPr family phosphocarrier protein -
F4V40_RS22080 4165303..4166163 + 861 WP_001131788.1 class II aldolase -
F4V40_RS22085 4166213..4166572 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F4V40_RS22090 4166569..4166844 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
F4V40_RS22095 4166917..4168041 - 1125 WP_001301659.1 ABC transporter permease -
F4V40_RS22100 4168041..4170776 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
F4V40_RS22105 4170773..4171840 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T140263 WP_001259386.1 NZ_CP044145:c4166844-4166569 [Escherichia coli O157]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T140263 NZ_CP059065:c857032-856748 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT140263 WP_000593555.1 NZ_CP044145:c4166572-4166213 [Escherichia coli O157]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT140263 NZ_CP059065:c856751-856458 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References