Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4123613..4124413 Replicon chromosome
Accession NZ_CP044145
Organism Escherichia coli O157 strain AR-0428

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag F4V40_RS21840 Protein ID WP_000342448.1
Coordinates 4123613..4124140 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag F4V40_RS21845 Protein ID WP_001277108.1
Coordinates 4124147..4124413 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V40_RS21815 4118688..4119455 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
F4V40_RS21820 4119452..4120729 - 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
F4V40_RS21825 4120726..4121652 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
F4V40_RS21830 4121700..4122809 - 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
F4V40_RS21835 4123233..4123616 + 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
F4V40_RS21840 4123613..4124140 - 528 WP_000342448.1 GNAT family N-acetyltransferase Toxin
F4V40_RS21845 4124147..4124413 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
F4V40_RS21850 4124563..4125666 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
F4V40_RS21855 4125937..4126791 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
F4V40_RS21860 4127036..4128094 - 1059 WP_001042018.1 permease-like cell division protein FtsX -
F4V40_RS21865 4128087..4128755 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T140262 WP_000342448.1 NZ_CP044145:c4124140-4123613 [Escherichia coli O157]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T140262 NZ_CP059065:c856751-856458 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT140262 WP_001277108.1 NZ_CP044145:c4124413-4124147 [Escherichia coli O157]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT140262 NZ_CP059065:c857032-856748 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References