Detailed information of TA system TA140261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PsyrTA/-
• Location: 4098273..4100601
• Replicon: chromosome
• Accession: NZ_CP044145
• Organism: Escherichia coli O157 strain AR-0428

Toxin (Protein)

• Gene name: PsyrT
• Uniprot ID: A0A6N0MHC8
• Locus tag: F4V40_RS21715
• Protein ID: WP_000532146.1
• Coordinates: 4099627..4100601 (-)
• Length: 325 a.a.

Antitoxin (Protein)

• Gene name: PsyrA
• Uniprot ID: -
• Locus tag: F4V40_RS21710
• Protein ID: WP_001303704.1
• Coordinates: 4098273..4099568 (-)
• Length: 432 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V40_RS21695	4093880..4096066	-	2187	WP_001283734.1	1,4-alpha-glucan branching enzyme	-
F4V40_RS29655	4096224..4096265	-	42	WP_211180509.1	protein YhgO	-
F4V40_RS21700	4096339..4097442	-	1104	WP_000799956.1	aspartate-semialdehyde dehydrogenase	-
F4V40_RS21705	4097634..4098227	+	594	WP_001002544.1	NAAT family transporter YhgN	-
F4V40_RS21710	4098273..4099568	-	1296	WP_001303704.1	DNA-protecting protein DprA	Antitoxin
F4V40_RS21715	4099627..4100601	-	975	WP_000532146.1	ATP-dependent DNA helicase	Toxin
F4V40_RS21720	4100605..4101006	-	402	WP_000502498.1	ATP-dependent DNA helicase	-
F4V40_RS21725	4100991..4101599	-	609	WP_000410808.1	ATP-dependent DNA helicase	-
F4V40_RS21730	4101739..4103079	-	1341	WP_000210111.1	gluconate transporter	-
F4V40_RS21735	4103083..4103610	-	528	WP_000108330.1	gluconokinase	-
F4V40_RS21740	4103749..4104744	-	996	WP_000730263.1	gluconate operon transcriptional repressor GntR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• DNA recombination-mediator protein A (115-429)

Sequences

Toxin

Protein

• Length: 325 a.a.
• Molecular weight: 36460.93 Da
• Isoelectric Point: 9.2667

>T140261 WP_000532146.1 NZ_CP044145:c4100601-4099627 [Escherichia coli O157]
VGRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQRTNLRYGQIEKALKLLVAENP
SPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDI
DSGLLHAANLFIKHADLSLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGYSGWGKQVAQEKKTGRFSDELVEAC
AEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFESTSTWFLILPGDWRRNLAYLLLMPLKKSWTIHRRKCSKTVSTSVKIS
TGRL

Nucleotide

• Length: 975 bp

>T140261 NZ_CP059064:c857032-856748 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin

Protein

• Length: 432 a.a.
• Molecular weight: 47053.95 Da
• Isoelectric Point: 7.2590

>AT140261 WP_001303704.1 NZ_CP044145:c4099568-4098273 [Escherichia coli O157]
MDADSSNSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLLTSDFSRAAASKYKPLSNSEWGKFALWLKHQRISPAE
LLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLI
AGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENG
ASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESISIERLASCT
GLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Nucleotide

• Length: 1296 bp

>AT140261 NZ_CP059064:c856751-856458 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Structures

Toxin

• AlphaFold DB: A0A6N0MHC8