Detailed information of TA system TA140247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1997132..1997603
• Replicon: chromosome
• Accession: NZ_CP044145
• Organism: Escherichia coli O157 strain AR-0428

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: F4V40_RS10840
• Protein ID: WP_001303511.1
• Coordinates: 1997325..1997603 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: F4V40_RS10835
• Protein ID: WP_001302048.1
• Coordinates: 1997132..1997323 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V40_RS10795	1992339..1992923	-	585	WP_000206793.1	DUF551 domain-containing protein	-
F4V40_RS10800	1992980..1993375	-	396	WP_001118159.1	DUF977 family protein	-
F4V40_RS10805	1993391..1994160	-	770	Protein_2030	DUF1627 domain-containing protein	-
F4V40_RS10810	1994186..1994926	-	741	WP_000788938.1	ATP-binding protein	-
F4V40_RS10815	1994933..1995895	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
F4V40_RS10820	1995918..1996343	-	426	WP_000693943.1	Rha family transcriptional regulator	-
F4V40_RS10825	1996340..1996642	-	303	WP_000172738.1	transcriptional regulator	-
F4V40_RS10830	1996740..1997111	+	372	WP_001169686.1	hypothetical protein	-
F4V40_RS10835	1997132..1997323	+	192	WP_001302048.1	hypothetical protein	Antitoxin
F4V40_RS10840	1997325..1997603	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F4V40_RS10845	1997956..1998255	+	300	WP_001240334.1	hypothetical protein	-
F4V40_RS10850	1998327..1998545	+	219	WP_001171930.1	hypothetical protein	-
F4V40_RS10860	1999114..1999302	+	189	WP_000449192.1	cell division inhibition protein DicB	-
F4V40_RS10865	1999299..1999490	+	192	WP_001090200.1	DUF1482 family protein	-
F4V40_RS10870	1999583..2002054	+	2472	WP_000048458.1	exonuclease	-
F4V40_RS10875	2002127..2002378	+	252	WP_000005552.1	excisionase family protein	-
F4V40_RS10880	2002413..2002597	+	185	Protein_2044	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1959311..2005890	46579

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T140247 WP_001303511.1 NZ_CP044145:1997325-1997603 [Escherichia coli O157]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T140247 NZ_CP059047:2474298-2474516 [Raoultella ornithinolytica]
ATGTCTGGTGAACCATTAACCAAAACAGACTATTTGATGCGCTTACGTCGCTGTCAGACAATTGATACGCTGGAACGCGT
AATTGAAAAAAACAAATATGAACTTTCAGATAACGAACTGGCTGTATTTTACTCAGCGGCCGACCACCGTCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAAATCCCGACATCGGTGTGGAAGTTCATTCGCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT140247 WP_001302048.1 NZ_CP044145:1997132-1997323 [Escherichia coli O157]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT140247 NZ_CP059047:2473897-2474271 [Raoultella ornithinolytica]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCGCAGCTTCGATTTCTTTGCGAAACCCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGTAACCACGGGTGGGTGAATGATCCAACGTCGGCTATCAATCTTCAACTTAATGAATTGATCGAAC
ACATAGCCACGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAACTGATCGCTCAGCTGGACGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGCATTAACACGGCCGATCTACAGAAATGGCGCAAATCGGGAAATCGGTT
ATTCCGATGTTTTGTCAACGCCAGTCGGGAAAATCCGGCAAGTCTTTCTTGTTAG

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0