Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1903638..1904276 Replicon chromosome
Accession NZ_CP044145
Organism Escherichia coli O157 strain AR-0428

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag F4V40_RS10330 Protein ID WP_000813794.1
Coordinates 1904100..1904276 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag F4V40_RS10325 Protein ID WP_001270286.1
Coordinates 1903638..1904054 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V40_RS10305 1898790..1899731 - 942 WP_001251320.1 ABC transporter permease -
F4V40_RS10310 1899732..1900745 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
F4V40_RS10315 1900763..1901908 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
F4V40_RS10320 1902153..1903559 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
F4V40_RS10325 1903638..1904054 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
F4V40_RS10330 1904100..1904276 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
F4V40_RS10335 1904498..1904728 + 231 WP_000494244.1 YncJ family protein -
F4V40_RS10340 1904820..1906781 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
F4V40_RS10345 1906854..1907390 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
F4V40_RS10350 1907443..1908654 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T140246 WP_000813794.1 NZ_CP044145:c1904276-1904100 [Escherichia coli O157]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T140246 NZ_CP059047:c1101539-1100208 [Raoultella ornithinolytica]
ATGGCGACTCTCGTGACATGGATGAACAATGAGCGGGTCGGCGAGCTAATCAAGCAGGCGAACGGCGCACATCTCTTTCG
CTATGAAGAGGCGTGGATGCGCAATCCGCGCGCCAGACCGCTCTCACTGTCGCTTCCGCTGCAGTACGGTAACATCACCG
ACGAGGCGGTATTTAACTACTTTGACAATCTGCTGCCGGACAGTCCGCGGGTACGGGACCGCATTGTTAAGCGCTATCAG
GCGCGTTCAAAGCAGCCGTTTGATCTGCTGGCCGAGATCGGACGGGACAGCGTCGGGGCGGTAACGTTACTCCCGCCAGG
AGAAAATGTGCCCCGCGGGGCGTTGAGCTGGGAGCCGCTGGATAACCACGCTCTGGATGCGTTACTGACGGCCTACCAGT
CGGATATACCGCTCGGAATGGTCAACGAACACGATGATTTCAGGATCTCGGTGGCCGGGGCCCAGGAGAAAACCGCGCTG
CTGAAAATCGGCGAGCAGTGGTGTATCCCGACGGGTACCACGCCGACTACCCACATCATTAAACTGCCCATCGGCGAGAT
CAAACAGCCGGATGCGGTGCTGGATCTCAGCGAAAGCGTCGATAACGAATATTTGTGTCTGGCGCTGGCCCGGGCGCTGG
GTTTTGCCGTTCCGCCCGCCAGCATTATCCAGACAGCCAGTATCAGGGCGCTGGCGGTGGAGCGTTTCGACCGTCGCTGG
GCGCAGGAGAAAACGGTGCTGCTGCGTTTGCCGCAGGAAGATCTGTGTCAGGCCTTCGGCATTCCGTCATCGATTAAGTA
TGAGTCCGACGGCGGGCCGGGCATTCGGGCAATTATGGCTTTTCTGGTTGGCTCCAGCGAGGCACTCGAGGATCGCTATA
ACTTTATGAAGTTTATGGTGTTCCAGTGGCTGATTGGCGCAACCGATGGCCACGCCAAAAACTTCTCGATTTTCCTCCAG
CCGGGCGGCAGCTACCGCTTAACCCCGTTCTACGACATTATCTCCGCATTTCCGCTGCTTGGCGGAAAGGGACTGCACCT
GAGCGATCTCAAGCTGTCGATGAGTCTGAAGGCCAGTAAAGGACGTAAAACGGAGATCCAGACGCTCTATCCGCGTCACT
TTCTCGCCACGGCTAAAGAGGTAGGGTTTGCCCAACAGCAGATGCTGGAGATTCTGCGCTATTTTGTCGATAACGTTCCC
CGCGCGGTTGAGGCGGTAAAGGGGACTTTACCGCCTGACTTTTCACAACCGGTATATGAGGCGATCACCGATCGCTTACT
GTGGGTCCATTCACGCTTGCAGAACGCAATGGCGGCGTCTCCGTCGGGCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT140246 WP_001270286.1 NZ_CP044145:c1904054-1903638 [Escherichia coli O157]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT140246 NZ_CP059047:c1101811-1101539 [Raoultella ornithinolytica]
ATGAGCCCTTCACCAACGATATACAGCCCGGTTCAGCTGGCAAACTATCTCAAGCTGGTGCGCCAGAAAAACCAGTGGAC
GCAGAGCGAGCTGGCTCAGCGTATGGGGATTAAACAGGCCACCGTCTCTAACTTTGAAAATCATCCCGAGAAAACGACGT
TAACCACGGTGTTTAAAATTTTACAGTCACTGGAACTGTCGATGGCGATTTGCGAAAAAAATGCGCCGGATACCGACGAT
AACCGTGATAACCCGCGGGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References