Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 255128..255746 Replicon chromosome
Accession NZ_CP044145
Organism Escherichia coli O157 strain AR-0428

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag F4V40_RS01260 Protein ID WP_001291435.1
Coordinates 255128..255346 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag F4V40_RS01265 Protein ID WP_000344800.1
Coordinates 255372..255746 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V40_RS01225 250415..250987 + 573 WP_000779831.1 YbaY family lipoprotein -
F4V40_RS01230 251020..251331 - 312 WP_000409911.1 MGMT family protein -
F4V40_RS01240 251710..252063 + 354 WP_000878140.1 DUF1428 domain-containing protein -
F4V40_RS01245 252105..253655 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
F4V40_RS01250 253819..254289 - 471 WP_000136192.1 YlaC family protein -
F4V40_RS01255 254405..254956 - 552 WP_000102553.1 maltose O-acetyltransferase -
F4V40_RS01260 255128..255346 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
F4V40_RS01265 255372..255746 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
F4V40_RS01270 256292..259441 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
F4V40_RS01275 259464..260657 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T140238 WP_001291435.1 NZ_CP044145:c255346-255128 [Escherichia coli O157]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T140238 NZ_CP059045:c2789815-2789453 [Cronobacter sakazakii]
ATGGCATGGACGGTGATTTTTACCGATCGTTTTAACGACTGGTATCAACAGCAGCCCGAAGGATTACAGGATCGTATCGC
CGCGCTCTTATGGAACTTACGTTATTCCGGCCCGCTTGTCGGACGCCCGCTGGTCGATACGGTGAAAGGTTCGCGTTATC
CCAACCTGAAAGAGCTACGTGTGCAGTATGGCGGCGAACCCTGGCGCGTTTTTTTTGCGTTTGACGCTGAACGTCAGGCC
GTTGTGCTCTGCGCAGGCAATAAACGGAGCCAGAAACATTTTTATGACGCCTTAATCAGGCAGGCTGAAGAAGAATTCTT
CCTGCATGTGCAAGCGATGGAGAGACGAAATGAAAACTCTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT140238 WP_000344800.1 NZ_CP044145:c255746-255372 [Escherichia coli O157]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT140238 NZ_CP059045:c2789466-2789170 [Cronobacter sakazakii]
ATGAAAACTCTTGATGACGTAATGGCGGGCTTTTCCCCGGAACGGCAGGCAGAAATCCTGCGCATGGCAGACGAAATCGC
GCTGGAGCACGGCCTGCCGCGCATTCGCGAAGCGCGGGCGTTTTCGCAGCAGCAACTGGCGGAGATAATGGGCGTGACGC
AGCCCGCTATCGCCGCCATTGAGCAGCGCGGTAAGGAGATTAAACTGCTGACGCTCAAGCGCTACGTCGAAGCGCTGGGC
GGCAAGCTCTCTTTACTGATAGAGCTGCCCGAAGGCAGCAAAGTGATCCCCGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References