Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 52907..53466 Replicon plasmid pAR-0429-2
Accession NZ_CP044144
Organism Escherichia coli O157 strain AR-0429

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag F4V41_RS29130 Protein ID WP_000421248.1
Coordinates 53191..53466 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag F4V41_RS29125 Protein ID WP_001178089.1
Coordinates 52907..53191 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V41_RS29070 48099..48659 + 561 WP_000840472.1 fertility inhibition protein FinO -
F4V41_RS29075 48792..49004 + 213 WP_001302189.1 ANR family transcriptional regulator -
F4V41_RS29080 49249..49710 + 462 WP_001233853.1 thermonuclease family protein -
F4V41_RS29085 49756..49965 + 210 WP_001302200.1 hemolysin expression modulator Hha -
F4V41_RS29090 50003..50341 + 339 WP_000766796.1 hypothetical protein -
F4V41_RS29095 50581..50835 + 255 WP_000083831.1 replication regulatory protein RepA -
F4V41_RS29105 51071..51145 + 75 WP_001370046.1 RepA leader peptide Tap -
F4V41_RS29110 51138..51995 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
F4V41_RS29125 52907..53191 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
F4V41_RS29130 53191..53466 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F4V41_RS29135 53561..53767 + 207 WP_001105064.1 hypothetical protein -
F4V41_RS29140 53867..54082 - 216 Protein_62 transposase -
F4V41_RS29145 54126..54692 - 567 Protein_63 IS3-like element IS600 family transposase -
F4V41_RS29150 54692..55108 - 417 Protein_64 IS91 family transposase -
F4V41_RS29155 55108..55293 - 186 WP_001302179.1 hypothetical protein -
F4V41_RS29160 55454..57679 + 2226 WP_032161295.1 catalase/peroxidase KatP -
F4V41_RS29165 57723..58112 + 390 WP_001172748.1 cytochrome b562 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyB / hlyD / hlyD / hlyD / toxB / toxB / toxB / toxB / toxB / toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB 1..92690 92690


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T140237 WP_000421248.1 NZ_CP044144:53191-53466 [Escherichia coli O157]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T140237 NZ_CP059045:c2529645-2529542 [Cronobacter sakazakii]
GGCAAGGCGACTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT140237 WP_001178089.1 NZ_CP044144:52907-53191 [Escherichia coli O157]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT140237 NZ_CP059045:2529539-2529707 [Cronobacter sakazakii]
GATAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGG
CATTAATGCAGGCTCAGTCGCCTTGCCTTTTAAGAATAGATGACGACACCAGCTTTTCCAGTTTGCGGCTAAAGTCGCAG
CAAAAAGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References