Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 7302..7827 Replicon plasmid pAR-0429-2
Accession NZ_CP044144
Organism Escherichia coli O157 strain AR-0429

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag F4V41_RS28820 Protein ID WP_001159868.1
Coordinates 7522..7827 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag F4V41_RS28815 Protein ID WP_000813634.1
Coordinates 7302..7520 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V41_RS28780 2585..2815 + 231 WP_010891288.1 hypothetical protein -
F4V41_RS28785 2936..3676 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
F4V41_RS28790 3961..4938 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
F4V41_RS28800 5346..5546 - 201 WP_000708307.1 hypothetical protein -
F4V41_RS28805 5543..6163 - 621 WP_001248529.1 hypothetical protein -
F4V41_RS28810 6160..6843 - 684 WP_010891291.1 DNA-binding protein -
F4V41_RS28815 7302..7520 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F4V41_RS28820 7522..7827 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
F4V41_RS28825 7828..8633 + 806 Protein_11 site-specific integrase -
F4V41_RS28840 10531..10869 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
F4V41_RS28845 11457..12623 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyB / hlyD / hlyD / hlyD / toxB / toxB / toxB / toxB / toxB / toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB 1..92690 92690


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T140236 WP_001159868.1 NZ_CP044144:7522-7827 [Escherichia coli O157]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T140236 NZ_CP059045:c1092232-1092014 [Cronobacter sakazakii]
ATGACGACCAAACCATTAACCAAAACCGATTATTTAATGCGACTGAGACGTTGTCAGACAATTGACACGCTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTATCCGACAACGAACTGGCGGTATTTTATTCAGCGGCGGATCATCGTCTGGCTGAAC
TGACGATGAATAAGCTCTACGATAAAATTCCGACCGCCGTGTGGAAGTTCGTTCGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT140236 WP_000813634.1 NZ_CP044144:7302-7520 [Escherichia coli O157]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT140236 NZ_CP059045:c1092633-1092259 [Cronobacter sakazakii]
ATGGACGAATACTCACCAAAACGGCATGATATAGCGCAGCTTAAATTTCTTTGCGAAAGCCTGTATCATGATTGCCTTGC
CACGCTCGGCGAAAACCAGCATGGCTGGGTTAACGACCCCACTTCTGCCGTTAACCTGCAACTCAACGACTTGATTGAGC
ATATTGCCAGCTTCGGCCTGAATTATAAAATTAAGCATGAAGATGACGCAGCTCTCATTGAGCAATTAGATGAATATCTG
GATGATACCTTTATGCTGTTCAGCAATTACGGTATCAATGCGCAAGATTTACAAAAATGGCGCAAGTCCGGAAACAGGCT
TTTCCGGTGTTTCGTAAACGCCAGCAAAGAAAACCCTGTAAGCTTGTCTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References