Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3480763..3481417 Replicon chromosome
Accession NZ_CP044143
Organism Escherichia coli O157 strain AR-0429

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag F4V41_RS18295 Protein ID WP_000244781.1
Coordinates 3481010..3481417 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag F4V41_RS18290 Protein ID WP_000354046.1
Coordinates 3480763..3481029 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V41_RS18270 3476851..3478284 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
F4V41_RS18275 3478329..3478640 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
F4V41_RS18280 3478804..3479463 + 660 WP_000250274.1 hemolysin III family protein -
F4V41_RS18285 3479540..3480520 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
F4V41_RS18290 3480763..3481029 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
F4V41_RS18295 3481010..3481417 + 408 WP_000244781.1 protein YgfX Toxin
F4V41_RS18300 3481457..3481978 - 522 WP_001055874.1 flavodoxin FldB -
F4V41_RS18305 3482090..3482986 + 897 WP_000806640.1 site-specific tyrosine recombinase XerD -
F4V41_RS18310 3483011..3483721 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
F4V41_RS18315 3483727..3485460 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T140224 WP_000244781.1 NZ_CP044143:3481010-3481417 [Escherichia coli O157]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T140224 NZ_CP059043:3212267-3212596 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT140224 WP_000354046.1 NZ_CP044143:3480763-3481029 [Escherichia coli O157]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT140224 NZ_CP059043:3211929-3212246 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References