Detailed information of TA system TA140221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2991269..2992023
• Replicon: chromosome
• Accession: NZ_CP044143
• Organism: Escherichia coli O157 strain AR-0429

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: F4V41_RS15750
• Protein ID: WP_001301452.1
• Coordinates: 2991269..2991754 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: F4V41_RS15755
• Protein ID: WP_000801912.1
• Coordinates: 2991745..2992023 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V41_RS15730	2986435..2987193	+	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-
F4V41_RS15735	2987175..2988773	-	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
F4V41_RS15740	2988961..2990187	+	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
F4V41_RS15745	2990191..2991219	+	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
F4V41_RS15750	2991269..2991754	-	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
F4V41_RS15755	2991745..2992023	-	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
F4V41_RS15760	2992090..2994795	-	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T140221 WP_001301452.1 NZ_CP044143:c2991754-2991269 [Escherichia coli O157]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T140221 NZ_CP059043:c2521725-2521471 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT140221 WP_000801912.1 NZ_CP044143:c2992023-2991745 [Escherichia coli O157]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT140221 NZ_CP059043:c2521973-2521722 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9