Detailed information of TA system TA140218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2899896..2900539
• Replicon: chromosome
• Accession: NZ_CP044143
• Organism: Escherichia coli O157 strain AR-0429

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: F4V41_RS15280
• Protein ID: WP_032161460.1
• Coordinates: 2899896..2900183 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: F4V41_RS15285
• Protein ID: WP_000593555.1
• Coordinates: 2900180..2900539 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V41_RS15265	2894913..2895980	+	1068	WP_000361458.1	HlyD family secretion protein	-
F4V41_RS15270	2895977..2898712	+	2736	WP_000149107.1	ribosome-associated ATPase/putative transporter RbbA	-
F4V41_RS15275	2898712..2899836	+	1125	WP_001301659.1	ABC transporter permease	-
F4V41_RS15280	2899896..2900183	+	288	WP_032161460.1	type II toxin-antitoxin system HicA family toxin	Toxin
F4V41_RS15285	2900180..2900539	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
F4V41_RS15290	2900589..2901449	-	861	WP_001131788.1	class II aldolase	-
F4V41_RS15295	2901481..2901750	-	270	WP_000933060.1	HPr family phosphocarrier protein	-
F4V41_RS15300	2901740..2903248	-	1509	WP_001301773.1	carbohydrate kinase	-
F4V41_RS15305	2903241..2904599	-	1359	WP_001302220.1	PTS galactitol transporter subunit IIC	-
F4V41_RS15310	2904676..2904957	-	282	WP_000084025.1	PTS sugar transporter subunit IIB	-
F4V41_RS15315	2904954..2905427	-	474	WP_001161553.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (34-84)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10502.00 Da
• Isoelectric Point: 7.7845

>T140218 WP_032161460.1 NZ_CP044143:2899896-2900183 [Escherichia coli O157]
IRTCHENQGTGFTEETKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGA
VESVRDWLLSIGVKP

Nucleotide

• Length: 288 bp

>T140218 NZ_CP059043:c2078669-2078382 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT140218 WP_000593555.1 NZ_CP044143:2900180-2900539 [Escherichia coli O157]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT140218 NZ_CP059043:c2078908-2078669 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6