Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1744933..1745483 Replicon chromosome
Accession NZ_CP044143
Organism Escherichia coli O157 strain AR-0429

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag F4V41_RS09960 Protein ID WP_001160975.1
Coordinates 1744933..1745247 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag F4V41_RS09965 Protein ID WP_000125566.1
Coordinates 1745250..1745483 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V41_RS09935 1740608..1741894 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
F4V41_RS09940 1741894..1742883 + 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
F4V41_RS09945 1742880..1743701 + 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
F4V41_RS09950 1743704..1744081 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
F4V41_RS09955 1744088..1744936 + 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
F4V41_RS09960 1744933..1745247 - 315 WP_001160975.1 CcdB family protein Toxin
F4V41_RS09965 1745250..1745483 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
F4V41_RS09970 1745569..1746048 - 480 WP_000624372.1 type 3 dihydrofolate reductase -
F4V41_RS09975 1746240..1748102 - 1863 WP_000377184.1 glutathione-regulated potassium-efflux system protein KefC -
F4V41_RS09980 1748095..1748625 - 531 WP_000600700.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
F4V41_RS09985 1748733..1750064 - 1332 WP_001183177.1 MFS transporter -
F4V41_RS09990 1750122..1750409 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T140213 WP_001160975.1 NZ_CP044143:c1745247-1744933 [Escherichia coli O157]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T140213 NZ_CP059043:c1702707-1702600 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT140213 WP_000125566.1 NZ_CP044143:c1745483-1745250 [Escherichia coli O157]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT140213 NZ_CP059043:1702754-1702820 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References