Detailed information of TA system TA140205

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 287892..288690
• Replicon: chromosome
• Accession: NZ_CP044143
• Organism: Escherichia coli O157 strain AR-0429

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0V7WHV8
• Locus tag: F4V41_RS02710
• Protein ID: WP_001506713.1
• Coordinates: 287892..288269 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A093DMQ4
• Locus tag: F4V41_RS02715
• Protein ID: WP_001280952.1
• Coordinates: 288316..288690 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V41_RS02670	282899..283390	-	492	WP_001301587.1	DUF1097 domain-containing protein	-
F4V41_RS02675	283492..284046	-	555	WP_001001917.1	molecular chaperone YcdY	-
F4V41_RS02680	284070..284807	-	738	WP_000283664.1	zinc-binding phosphatase	-
F4V41_RS02685	284862..285800	-	939	WP_000351284.1	glyoxylate/hydroxypyruvate reductase GhrA	-
F4V41_RS02695	286271..287113	-	843	WP_001688445.1	DUF4942 domain-containing protein	-
F4V41_RS02700	287198..287395	-	198	WP_000839282.1	DUF957 domain-containing protein	-
F4V41_RS02705	287407..287895	-	489	WP_001470575.1	hypothetical protein	-
F4V41_RS02710	287892..288269	-	378	WP_001506713.1	TA system toxin CbtA family protein	Toxin
F4V41_RS02715	288316..288690	-	375	WP_001280952.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F4V41_RS02720	288853..289074	-	222	WP_001220316.1	DUF987 domain-containing protein	-
F4V41_RS02725	289143..289619	-	477	WP_001186714.1	RadC family protein	-
F4V41_RS02730	289635..290120	-	486	WP_001470565.1	antirestriction protein	-
F4V41_RS02735	290212..291030	-	819	WP_151121003.1	DUF945 domain-containing protein	-
F4V41_RS02745	291370..292440	-	1071	WP_000102643.1	patatin-like phospholipase family protein	-
F4V41_RS02750	292437..293342	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgB / csgD / csgE / csgF / csgG	279281..290120	10839

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14178.26 Da
• Isoelectric Point: 7.8045

>T140205 WP_001506713.1 NZ_CP044143:c288269-287892 [Escherichia coli O157]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYITVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T140205 NZ_CP059042:4537-4824 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13719.50 Da
• Isoelectric Point: 6.6249

>AT140205 WP_001280952.1 NZ_CP044143:c288690-288316 [Escherichia coli O157]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Nucleotide

• Length: 375 bp

>AT140205 NZ_CP059042:4811-5125 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTAAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0V7WHV8

Antitoxin

• AlphaFold DB: A0A093DMQ4