Detailed information of TA system TA140199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4515591..4516229
• Replicon: chromosome
• Accession: NZ_CP044140
• Organism: Escherichia coli O157 strain AR-0430

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: F4V42_RS23655
• Protein ID: WP_000813794.1
• Coordinates: 4515591..4515767 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: F4V42_RS23660
• Protein ID: WP_001270286.1
• Coordinates: 4515813..4516229 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V42_RS23635	4511213..4512424	-	1212	WP_071525382.1	BenE family transporter YdcO	-
F4V42_RS23640	4512477..4513013	+	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
F4V42_RS23645	4513086..4515047	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
F4V42_RS23650	4515139..4515369	-	231	WP_000494244.1	YncJ family protein	-
F4V42_RS23655	4515591..4515767	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
F4V42_RS23660	4515813..4516229	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
F4V42_RS23665	4516308..4517714	+	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
F4V42_RS23670	4517959..4519104	+	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
F4V42_RS23675	4519122..4520135	+	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
F4V42_RS23680	4520136..4521077	+	942	WP_001251320.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T140199 WP_000813794.1 NZ_CP044140:4515591-4515767 [Escherichia coli O157]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T140199 NZ_CP059035:1800271-1800597 [Pasteurella multocida subsp. multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGCG
ATTTGCGAAGTCAACCAGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAATATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT140199 WP_001270286.1 NZ_CP044140:4515813-4516229 [Escherichia coli O157]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT140199 NZ_CP059035:1800719-1801045 [Pasteurella multocida subsp. multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCGAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGTCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA

Structures

Toxin

• PDB: 6HPB