Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4515591..4516229 Replicon chromosome
Accession NZ_CP044140
Organism Escherichia coli O157 strain AR-0430

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag F4V42_RS23655 Protein ID WP_000813794.1
Coordinates 4515591..4515767 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag F4V42_RS23660 Protein ID WP_001270286.1
Coordinates 4515813..4516229 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V42_RS23635 4511213..4512424 - 1212 WP_071525382.1 BenE family transporter YdcO -
F4V42_RS23640 4512477..4513013 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
F4V42_RS23645 4513086..4515047 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
F4V42_RS23650 4515139..4515369 - 231 WP_000494244.1 YncJ family protein -
F4V42_RS23655 4515591..4515767 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
F4V42_RS23660 4515813..4516229 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
F4V42_RS23665 4516308..4517714 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
F4V42_RS23670 4517959..4519104 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
F4V42_RS23675 4519122..4520135 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
F4V42_RS23680 4520136..4521077 + 942 WP_001251320.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T140199 WP_000813794.1 NZ_CP044140:4515591-4515767 [Escherichia coli O157]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T140199 NZ_CP059035:1800271-1800597 [Pasteurella multocida subsp. multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGCG
ATTTGCGAAGTCAACCAGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAATATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT140199 WP_001270286.1 NZ_CP044140:4515813-4516229 [Escherichia coli O157]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT140199 NZ_CP059035:1800719-1801045 [Pasteurella multocida subsp. multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCGAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGTCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References