Detailed information of TA system TA140198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 4422274..4422745
• Replicon: chromosome
• Accession: NZ_CP044140
• Organism: Escherichia coli O157 strain AR-0430

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: F4V42_RS23145
• Protein ID: WP_001303511.1
• Coordinates: 4422274..4422552 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: F4V42_RS23150
• Protein ID: WP_001302048.1
• Coordinates: 4422554..4422745 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V42_RS23110	4417499..4417750	-	252	WP_000005552.1	excisionase family protein	-
F4V42_RS23115	4417823..4420294	-	2472	WP_000048458.1	exonuclease	-
F4V42_RS23120	4420387..4420578	-	192	WP_001090200.1	DUF1482 family protein	-
F4V42_RS23125	4420575..4420763	-	189	WP_000449192.1	cell division inhibition protein DicB	-
F4V42_RS23135	4421332..4421550	-	219	WP_001171930.1	hypothetical protein	-
F4V42_RS23140	4421622..4421921	-	300	WP_001240334.1	hypothetical protein	-
F4V42_RS23145	4422274..4422552	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F4V42_RS23150	4422554..4422745	-	192	WP_001302048.1	hypothetical protein	Antitoxin
F4V42_RS23155	4422766..4423137	-	372	WP_001169686.1	hypothetical protein	-
F4V42_RS23160	4423235..4423537	+	303	WP_000172738.1	transcriptional regulator	-
F4V42_RS23165	4423534..4423959	+	426	WP_000693943.1	Rha family transcriptional regulator	-
F4V42_RS23170	4423982..4424944	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
F4V42_RS23175	4424951..4425691	+	741	WP_000788938.1	ATP-binding protein	-
F4V42_RS23180	4425717..4426486	+	770	Protein_4131	DUF1627 domain-containing protein	-
F4V42_RS23185	4426502..4426897	+	396	WP_001118159.1	DUF977 family protein	-
F4V42_RS23190	4426954..4427538	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4413987..4460556	46569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T140198 WP_001303511.1 NZ_CP044140:c4422552-4422274 [Escherichia coli O157]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T140198 NZ_CP059035:462845-463138 [Pasteurella multocida subsp. multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAAGTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTATGCATTCCTTAGGTATTAAACTTGTCCCAACGCACATCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT140198 WP_001302048.1 NZ_CP044140:c4422745-4422554 [Escherichia coli O157]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT140198 NZ_CP059035:462564-462848 [Pasteurella multocida subsp. multocida]
GTGGGTCGGTTAAAGGAGGTAAAAATTTTATCCGCCAAATCTGCTGTTCTTGCTCGTATTAACAAAGCAATGAATGGTAA
TTTTGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0