Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2309028..2309659 Replicon chromosome
Accession NZ_CP044140
Organism Escherichia coli O157 strain AR-0430

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag F4V42_RS12305 Protein ID WP_001259386.1
Coordinates 2309028..2309303 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag F4V42_RS12310 Protein ID WP_000593555.1
Coordinates 2309300..2309659 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V42_RS12290 2304032..2305099 + 1068 WP_000361458.1 HlyD family secretion protein -
F4V42_RS12295 2305096..2307831 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
F4V42_RS12300 2307831..2308955 + 1125 WP_001301659.1 ABC transporter permease -
F4V42_RS12305 2309028..2309303 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
F4V42_RS12310 2309300..2309659 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F4V42_RS12315 2309709..2310569 - 861 WP_001131788.1 class II aldolase -
F4V42_RS12320 2310601..2310870 - 270 WP_000933060.1 HPr family phosphocarrier protein -
F4V42_RS12325 2310860..2312368 - 1509 WP_001301773.1 carbohydrate kinase -
F4V42_RS12330 2312361..2313719 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
F4V42_RS12335 2313796..2314077 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
F4V42_RS12340 2314074..2314547 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2300172..2309659 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T140182 WP_001259386.1 NZ_CP044140:2309028-2309303 [Escherichia coli O157]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T140182 NZ_CP059003:c3693068-3692733 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT140182 WP_000593555.1 NZ_CP044140:2309300-2309659 [Escherichia coli O157]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT140182 NZ_CP059003:c3693316-3693068 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGAAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References