Detailed information of TA system TA140177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1157939..1158489
• Replicon: chromosome
• Accession: NZ_CP044140
• Organism: Escherichia coli O157 strain AR-0430

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: F4V42_RS06990
• Protein ID: WP_001160975.1
• Coordinates: 1157939..1158253 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: F4V42_RS06995
• Protein ID: WP_000125566.1
• Coordinates: 1158256..1158489 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V42_RS06965	1153614..1154900	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
F4V42_RS06970	1154900..1155889	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
F4V42_RS06975	1155886..1156707	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
F4V42_RS06980	1156710..1157087	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
F4V42_RS06985	1157094..1157942	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
F4V42_RS06990	1157939..1158253	-	315	WP_001160975.1	CcdB family protein	Toxin
F4V42_RS06995	1158256..1158489	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
F4V42_RS07000	1158575..1159054	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
F4V42_RS07005	1159246..1161108	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
F4V42_RS07010	1161101..1161631	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
F4V42_RS07015	1161739..1163070	-	1332	WP_001183177.1	MFS transporter	-
F4V42_RS07020	1163128..1163415	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T140177 WP_001160975.1 NZ_CP044140:c1158253-1157939 [Escherichia coli O157]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T140177 NZ_CP059003:1705595-1705849 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT140177 WP_000125566.1 NZ_CP044140:c1158489-1158256 [Escherichia coli O157]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT140177 NZ_CP059003:1705347-1705598 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9