Detailed information of TA system TA140173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 211522..212227
• Replicon: chromosome
• Accession: NZ_CP044140
• Organism: Escherichia coli O157 strain AR-0430

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: F4V42_RS02530
• Protein ID: WP_000539519.1
• Coordinates: 211522..211908 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: F4V42_RS02535
• Protein ID: WP_001280945.1
• Coordinates: 211898..212227 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V42_RS02510	207526..208152	+	627	WP_001295292.1	glutathione S-transferase GstB	-
F4V42_RS02515	208149..209264	-	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
F4V42_RS02520	209375..209758	-	384	WP_000497137.1	biofilm formation regulator BssR	-
F4V42_RS02525	209971..211296	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
F4V42_RS02530	211522..211908	+	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F4V42_RS02535	211898..212227	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
F4V42_RS02540	212297..213625	-	1329	WP_000086905.1	GGDEF domain-containing protein	-
F4V42_RS02545	213633..215981	-	2349	WP_000950320.1	EAL domain-containing protein	-
F4V42_RS02550	216159..217070	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T140173 WP_000539519.1 NZ_CP044140:211522-211908 [Escherichia coli O157]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T140173 NZ_CP059003:c1090477-1089935 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT140173 WP_001280945.1 NZ_CP044140:211898-212227 [Escherichia coli O157]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT140173 NZ_CP059003:c1090772-1090461 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGCCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA