Detailed information of TA system TA140171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 64493..65052
• Replicon: plasmid pAR-0430-3
• Accession: NZ_CP044139
• Organism: Escherichia coli O157 strain AR-0430

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: F4V42_RS01255
• Protein ID: WP_000421248.1
• Coordinates: 64493..64768 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: F4V42_RS01260
• Protein ID: WP_001178089.1
• Coordinates: 64768..65052 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V42_RS01220	59848..60144	-	297	WP_071527595.1	cytochrome B562	-
F4V42_RS01225	60280..62489	-	2210	Protein_58	catalase/peroxidase KatP	-
F4V42_RS01230	62666..62851	+	186	WP_001302179.1	hypothetical protein	-
F4V42_RS01235	62851..63267	+	417	Protein_60	IS91 family transposase	-
F4V42_RS01240	63267..63833	+	567	Protein_61	IS3-like element IS600 family transposase	-
F4V42_RS01245	63877..64092	+	216	Protein_62	transposase	-
F4V42_RS01250	64192..64398	-	207	WP_001105064.1	hypothetical protein	-
F4V42_RS01255	64493..64768	-	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F4V42_RS01260	64768..65052	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
F4V42_RS01275	65964..66821	-	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
F4V42_RS01280	66814..66888	-	75	WP_001370046.1	RepA leader peptide Tap	-
F4V42_RS01290	67124..67378	-	255	WP_000083831.1	replication regulatory protein RepA	-
F4V42_RS01295	67618..67956	-	339	WP_000766796.1	hypothetical protein	-
F4V42_RS01300	67994..68203	-	210	WP_001302200.1	hemolysin expression modulator Hha	-
F4V42_RS01305	68249..68710	-	462	WP_001233853.1	thermonuclease family protein	-
F4V42_RS01310	68955..69167	-	213	WP_001302189.1	ANR family transcriptional regulator	-
F4V42_RS01315	69300..69860	-	561	WP_000840472.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB / toxB / toxB / toxB	1..92728	92728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T140171 WP_000421248.1 NZ_CP044139:c64768-64493 [Escherichia coli O157]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T140171 NZ_CP059003:402355-402645 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT140171 WP_001178089.1 NZ_CP044139:c65052-64768 [Escherichia coli O157]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT140171 NZ_CP059003:402123-402365 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8