Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 17432..17957 Replicon plasmid pAR-0430-3
Accession NZ_CP044139
Organism Escherichia coli O157 strain AR-0430

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag F4V42_RS01000 Protein ID WP_001159868.1
Coordinates 17432..17737 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag F4V42_RS01005 Protein ID WP_000813634.1
Coordinates 17739..17957 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V42_RS00970 12637..13803 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
F4V42_RS00980 14390..14728 - 339 WP_071525396.1 RepB family plasmid replication initiator protein -
F4V42_RS00985 14690..15903 + 1214 WP_162829202.1 IS3 family transposase -
F4V42_RS00995 16625..17431 - 807 WP_000016989.1 site-specific integrase -
F4V42_RS01000 17432..17737 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
F4V42_RS01005 17739..17957 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F4V42_RS01010 18416..19099 + 684 WP_010891291.1 DNA-binding protein -
F4V42_RS01015 19096..19716 + 621 WP_001248529.1 hypothetical protein -
F4V42_RS01020 19713..19913 + 201 WP_000708307.1 hypothetical protein -
F4V42_RS01030 20321..21298 + 978 WP_000361615.1 RepB family plasmid replication initiator protein -
F4V42_RS01035 21583..22323 - 741 WP_001066920.1 tyrosine-type recombinase/integrase -
F4V42_RS01040 22444..22674 - 231 WP_010891288.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB / toxB / toxB / toxB 1..92728 92728


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T140170 WP_001159868.1 NZ_CP044139:c17737-17432 [Escherichia coli O157]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T140170 NZ_CP059003:390951-391301 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT140170 WP_000813634.1 NZ_CP044139:c17957-17739 [Escherichia coli O157]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT140170 NZ_CP059003:390706-390957 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References