Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 22584..23049 Replicon plasmid pAR-0430-2
Accession NZ_CP044138
Organism Escherichia coli O157 strain AR-0430

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag F4V42_RS00755 Protein ID WP_001230707.1
Coordinates 22584..22835 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag F4V42_RS00760 Protein ID WP_000801440.1
Coordinates 22822..23049 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V42_RS00730 17984..18850 - 867 WP_001353743.1 replication initiation protein -
F4V42_RS00735 20105..20440 + 336 WP_001147119.1 type II toxin-antitoxin system RelE/ParE family toxin -
F4V42_RS00740 20437..20786 + 350 Protein_25 DNA-binding protein -
F4V42_RS00745 21426..21941 + 516 WP_001025395.1 J domain-containing protein -
F4V42_RS00750 22096..22587 + 492 WP_151120042.1 hypothetical protein -
F4V42_RS00755 22584..22835 + 252 WP_001230707.1 hypothetical protein Toxin
F4V42_RS00760 22822..23049 + 228 WP_000801440.1 hypothetical protein Antitoxin
F4V42_RS00770 23503..23802 + 300 WP_000650311.1 hypothetical protein -
F4V42_RS00775 23812..24228 + 417 WP_012421208.1 tail-associated lysozyme -
F4V42_RS00780 24225..24536 + 312 WP_000627400.1 hypothetical protein -
F4V42_RS00785 24841..24972 + 132 WP_001353745.1 toxin-antitoxin system HicB family antitoxin -
F4V42_RS00795 25333..25836 + 504 WP_000781813.1 hypothetical protein -
F4V42_RS00800 25840..27069 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
F4V42_RS00805 27146..27439 + 294 WP_000134733.1 LuxR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..34677 34677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T140169 WP_001230707.1 NZ_CP044138:22584-22835 [Escherichia coli O157]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T140169 NZ_CP059003:285427-285600 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT140169 WP_000801440.1 NZ_CP044138:22822-23049 [Escherichia coli O157]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT140169 NZ_CP059003:285103-285399 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References