Detailed information of TA system TA140159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1570451..1571096
• Replicon: chromosome
• Accession: NZ_CP044136
• Organism: Proteus mirabilis strain ENT1157

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B4ET86
• Locus tag: F4W58_RS07425
• Protein ID: WP_004247035.1
• Coordinates: 1570911..1571096 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B4ET85
• Locus tag: F4W58_RS07420
• Protein ID: WP_004247034.1
• Coordinates: 1570451..1570864 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4W58_RS19105	1565994..1566152	-	159	WP_004247025.1	hypothetical protein	-
F4W58_RS07395	1566961..1567707	-	747	WP_017628402.1	methyltransferase domain-containing protein	-
F4W58_RS07405	1568557..1569135	+	579	WP_017628400.1	alpha/beta hydrolase	-
F4W58_RS07410	1569248..1569541	-	294	Protein_1427	LysE family transporter	-
F4W58_RS07415	1569855..1570355	-	501	WP_004247032.1	response regulator	-
F4W58_RS07420	1570451..1570864	-	414	WP_004247034.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
F4W58_RS07425	1570911..1571096	-	186	WP_004247035.1	type II toxin-antitoxin system HicA family toxin	Toxin
F4W58_RS07430	1571207..1571977	+	771	Protein_1431	DUF1073 domain-containing protein	-
F4W58_RS07435	1572536..1573087	-	552	WP_017628396.1	PH domain-containing protein	-
F4W58_RS07440	1573151..1573465	-	315	WP_017628395.1	hypothetical protein	-
F4W58_RS07450	1573872..1575605	-	1734	WP_017628394.1	LPS biosynthesis-modulating metalloenzyme YejM	-
F4W58_RS07455	1575629..1575856	-	228	WP_004247042.1	YejL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1565211..1580195	14984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 7049.30 Da
• Isoelectric Point: 10.9659

>T140159 WP_004247035.1 NZ_CP044136:c1571096-1570911 [Proteus mirabilis]
MKSSELIKLLEKNGWILDRIKGSHHQFTHPDFSFVVTVPHPRKDLKKGTLNQIIKSAKLKN

Nucleotide

• Length: 186 bp

>T140159 NZ_CP058991:c857032-856748 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15251.98 Da
• Isoelectric Point: 4.3830

>AT140159 WP_004247034.1 NZ_CP044136:c1570864-1570451 [Proteus mirabilis]
MLYPAFIEIDEDGSASGWFPDIDGCIFAGDSIEEAHADARSAIDAHFELLSEKGLDIPSPKSQQEHLINSSGDYNNGIWL
LVDVDMDKYDGRAERINITLPHRLLSRIDTIVKTNPEYGSRSGFIATATRKELQKNI

Nucleotide

• Length: 414 bp

>AT140159 NZ_CP058991:c856751-856458 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Structures

Toxin

• AlphaFold DB: B4ET86

Antitoxin

• AlphaFold DB: B4ET85