Detailed information of TA system TA140157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1322829..1323746
• Replicon: chromosome
• Accession: NZ_CP044133
• Organism: Bacillus velezensis strain FJAT-46737

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: F3129_RS06970
• Protein ID: WP_015239694.1
• Coordinates: 1323000..1323746 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: F3129_RS06965
• Protein ID: WP_003154807.1
• Coordinates: 1322829..1322999 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3129_RS06930	1318041..1319630	+	1590	WP_015239691.1	hypothetical protein	-
F3129_RS06935	1319643..1320068	+	426	WP_051483351.1	hypothetical protein	-
F3129_RS06940	1320073..1320270	+	198	WP_012117366.1	XkdX family protein	-
F3129_RS06945	1320327..1321088	+	762	WP_043021265.1	hypothetical protein	-
F3129_RS06950	1321140..1321403	+	264	WP_003154815.1	hemolysin XhlA family protein	-
F3129_RS06955	1321417..1321680	+	264	WP_003154813.1	phage holin	-
F3129_RS06960	1321694..1322572	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
F3129_RS06965	1322829..1322999	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
F3129_RS06970	1323000..1323746	-	747	WP_015239694.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
F3129_RS06975	1323854..1324852	-	999	WP_003154805.1	inorganic phosphate transporter	-
F3129_RS06980	1324865..1325482	-	618	WP_003154804.1	DUF47 domain-containing protein	-
F3129_RS06985	1325768..1327084	-	1317	WP_007407254.1	amino acid permease	-
F3129_RS06990	1327407..1328357	+	951	WP_150941166.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29135.61 Da
• Isoelectric Point: 4.7003

>T140157 WP_015239694.1 NZ_CP044133:c1323746-1323000 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T140157 NZ_CP058971:c1714119-1713859 [Micrococcus luteus]
ATGTCCATCAGCGCCAGTGAAGCCCGCAAGACCCTGTTCCCGCTCATCGAGCGAGTGAATGCGGACCGCGACGCCGTAGA
GATCGTCTCCCGCAAGGGGAATGCCGTCCTCATGCCCGCGGACGAGTACGCCGCCTGGCAGGAGACCGCCTACCTGTTCC
GCTCCCCGGCCAACGCCCGCCGGCTGCTGGACGCCTACGAGCGCGCCCGCGCAGGGAGCGTCGAGGAGCACGCTCTGGAC
CGCATGGACGGGGACGCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT140157 WP_003154807.1 NZ_CP044133:c1322999-1322829 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT140157 NZ_CP058971:c1713858-1713604 [Micrococcus luteus]
GTGCGCCTCGTGTGGGACCGCTCGGCCTGGGAGGACTACACGCACTGGCAGACCACGGACCGCAAGGTCCTCAAGCGGAT
CAACACCCTCATCGACGCCGCCCTGCGTGATCCCTTCGACGGGATCGGCAAGCCCGAGCAGCTGAAGTACGGCGCGCCCG
GGGCATGGTCCCGACGCATCACGGAGGAGCACAGGCTCGTCTACCTCGTCGACGGCGAGGACCTGATCATCCTCCAGGCG
AGGTATCACTACTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14