Detailed information of TA system TA140155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1336407..1337324
• Replicon: chromosome
• Accession: NZ_CP044132
• Organism: Bacillus amyloliquefaciens strain ZKY01

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: F5K02_RS06940
• Protein ID: WP_094032372.1
• Coordinates: 1336578..1337324 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: F5K02_RS06935
• Protein ID: WP_003154807.1
• Coordinates: 1336407..1336577 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F5K02_RS06895 (F5K02_06940)	1331620..1333209	+	1590	WP_053573489.1	hypothetical protein	-
F5K02_RS06900 (F5K02_06945)	1333222..1333647	+	426	WP_079892045.1	hypothetical protein	-
F5K02_RS06905 (F5K02_06950)	1333652..1333849	+	198	WP_012117366.1	XkdX family protein	-
F5K02_RS06910 (F5K02_06955)	1333906..1334667	+	762	WP_241458136.1	phage portal protein	-
F5K02_RS06915 (F5K02_06960)	1334719..1334982	+	264	WP_003154815.1	hemolysin XhlA family protein	-
F5K02_RS06920 (F5K02_06965)	1334996..1335259	+	264	WP_241458138.1	phage holin	-
F5K02_RS06925 (F5K02_06970)	1335273..1336151	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
F5K02_RS06935 (F5K02_06975)	1336407..1336577	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
F5K02_RS06940 (F5K02_06980)	1336578..1337324	-	747	WP_094032372.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
F5K02_RS06945 (F5K02_06985)	1337429..1338427	-	999	WP_020955696.1	inorganic phosphate transporter	-
F5K02_RS06950 (F5K02_06990)	1338440..1339057	-	618	WP_003154804.1	DUF47 domain-containing protein	-
F5K02_RS06955 (F5K02_06995)	1339343..1340659	-	1317	WP_094032373.1	amino acid permease	-
F5K02_RS06960 (F5K02_07000)	1340982..1341932	+	951	WP_094032374.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1305277..1339057	33780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.57 Da
• Isoelectric Point: 4.7755

>T140155 WP_094032372.1 NZ_CP044132:c1337324-1336578 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQADKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T140155 NZ_CP058970:c6252033-6251179 [Delftia acidovorans]
ATGACCCCCTCGGTAACAGCATGCATGGAGCAGCGCCTTCAGGCGCAGTTCTCCGAACACCTCATCGCCCTGCCCGGTGG
CGCTCAGGCCGCCTGGCGCGAGGCCGGCGACACGGCCTCGGACTTTGCCGTGGTGATGCTGCACGGCATCAGCTCGGGCG
CCGCCTCCTGGCTGGACGTGGCCCTGGCGCTGGCACCGCGCACGCGTGTGCTGGCCTGGGACGCGCCCGGCTATGGCCGC
TCCACGCCGCTGGCACCCGACGCGCCCACGGGCGCCGACTACGCCCGCATGCTGGAGCAGTCGCTGCAGGCGCTGGGCGT
GCGCCGCTGCCTGCTGGTGGGCCACTCGCTGGGTGCGCTCATGGGCACGCCCTGGGCAGTGCAGGCCGGTGCCGGGCGCG
TGCAGCAACTGGTGCTGATCAGCCCCGCTGGCGGCTATGGCGCACCCGGCATGGGCGAGCAGCAGGCCAAGGTACGCCAG
GGCCGTCTTGCGGCCCTGCAGGAAAAAGGCATCGAAGGCCTGGCCGCCGCCATCGACCAGCGCCTGGTCTCGCCCGCCGC
GCCTGAGCCCGTGCGCCAATGGGTGCGCTGGAACACGGCGCGCCTGCATGCTGCCGGCTACGCCCAGGCCGTGGAGCTGC
TGTGCGGCAGCGACTTGGGGCAGGCGGCCGGGCAACTGGCCATGCCGGTCGAGGTCTGGGTGGGCGAGCACGACGTGGTC
ACGCCGCCCGCCGCCTGCGCGGCCTGGGCCGCAAGGCTGGGCGCGCGGTACGTTACGCTTGCGGCAGCCGGCCATGCCTC
TGTCGTCGAACAACCTGCGGATGTCGCACAGAGGCTGGCCACCCTGCTGACCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT140155 WP_003154807.1 NZ_CP044132:c1336577-1336407 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT140155 NZ_CP058970:c6251156-6250350 [Delftia acidovorans]
ATGAGCGACACCTCCGAAACCCTCTCGAACGACAACGACCGCTACACCGTCCCCGCGCTGGAGCGCGGCCTGCGCCTGCT
GGCCTGCTTCACGCCTTCGCAGCCCGTGTGGAGCGCGCCCGACCTGGCGCGCACCCTGGCGCTGCCGCGTTCCACGGTGT
TCCGGATGCTGACGACGCTGGAGAACATGGGGTATCTGCAACGCTCGGGCACCGAGTACCGGCTGGGCCTGTCCGTGCTG
CGCCTGGGCTACGACTACCTGTCCACCCAGCCGCTGGCCCAGCTGGCCGAGCCCGTGCTGCAGGCGCTGTGCGAGCGCCT
GGGCTTCACCACCAACCTGGCGCTGCTGGACGGCACCTCCGTGGTCTACGTGGCGCGCATTGCGCCGGCCGGCGCCTTCC
AGGGCGCCGTGCGCGTGGGCTCGCGCCTGCCGGCCCATGCCACCGTGCTGGGCCGCGCCCTGTTGCAGGACATGGACGCA
GACCAGCTGCGAGAACTCTTCGGCGGCAGCGAACTGCCGCAATTCTCGGAAAGCACGCCGCGTGACGTGCAGCAACTGCA
GCAGCTGCTGCTGCAGGACCGCGAGCGCGGCTACGCCATGGGCGAGGGCTTCTACGAGCCCGGCGTCTCCAGCATTGCCG
CGCCCGTGCGCGATGCGCAGGGCCGCATCATCGCGGGCCTGGGCATGGCCGTGGCCTTTACCGAAATCGCACCCGGGCGC
ATGGCGCACTGGGTGGAACAGGTGCGCGAGGCCGCCGTCGAACTGACGGGCCACCTGCGCACCCAGCATCCTGGTTTTCT
GCGCTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14