Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 527669..528306 | Replicon | chromosome |
| Accession | NZ_CP044132 | ||
| Organism | Bacillus amyloliquefaciens strain ZKY01 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | F5K02_RS02525 | Protein ID | WP_003156187.1 |
| Coordinates | 527956..528306 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | F5K02_RS02520 | Protein ID | WP_003156188.1 |
| Coordinates | 527669..527950 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| F5K02_RS02500 (F5K02_02510) | 524034..524633 | - | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
| F5K02_RS02505 (F5K02_02515) | 524726..525091 | + | 366 | WP_007410229.1 | holo-ACP synthase | - |
| F5K02_RS02510 (F5K02_02520) | 525256..526263 | + | 1008 | WP_043021433.1 | outer membrane lipoprotein carrier protein LolA | - |
| F5K02_RS02515 (F5K02_02525) | 526380..527549 | + | 1170 | WP_094032115.1 | alanine racemase | - |
| F5K02_RS02520 (F5K02_02530) | 527669..527950 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| F5K02_RS02525 (F5K02_02535) | 527956..528306 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| F5K02_RS02530 (F5K02_02540) | 528424..529245 | + | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| F5K02_RS02535 (F5K02_02545) | 529250..529615 | + | 366 | WP_003156180.1 | RsbT antagonist protein RsbS | - |
| F5K02_RS02540 (F5K02_02550) | 529618..530019 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| F5K02_RS02545 (F5K02_02555) | 530031..531038 | + | 1008 | WP_007410233.1 | PP2C family protein-serine/threonine phosphatase | - |
| F5K02_RS02550 (F5K02_02560) | 531102..531431 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| F5K02_RS02555 (F5K02_02565) | 531428..531910 | + | 483 | WP_003156173.1 | anti-sigma B factor RsbW | - |
| F5K02_RS02560 (F5K02_02570) | 531876..532664 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| F5K02_RS02565 (F5K02_02575) | 532664..533266 | + | 603 | WP_007410234.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T140154 WP_003156187.1 NZ_CP044132:527956-528306 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T140154 NZ_CP058970:c5472553-5472152 [Delftia acidovorans]
ATGATCCTGCTCGACACCAACATCTGCATCTACATCATCAATGCCAAGCCCCCGGAGGTGCTGGCCCGCTTTCAGCAGTA
CCGCATGGGCGAGGTGGGATTGTGCAGCGTGGTTGCTGCCGAACTGGCCTTTGGCGTGGCCAAAAGCGGCTCGGCCCGTA
ACCGCCAGGCCCTGGAGATGTTTCTTGCCCCGCTCGCCATCCTCCCGTTTGACGAGGCGGCCGTGTGGGCCTATGGCGAC
CTGCGTGCCGATCTGGAGCGTCGAGGCAGCCCCATAGGCGCGCTTGACACCATGATCGCTGCCCACGCCCTCAGTCAGCG
GGCCCTGCTGGTCACCAACAACACCCGCGAATTCGCCAAGGTGCCCGGCCTGCAGTTGGACAACTGGGCTGTCGCTACCT
GA
ATGATCCTGCTCGACACCAACATCTGCATCTACATCATCAATGCCAAGCCCCCGGAGGTGCTGGCCCGCTTTCAGCAGTA
CCGCATGGGCGAGGTGGGATTGTGCAGCGTGGTTGCTGCCGAACTGGCCTTTGGCGTGGCCAAAAGCGGCTCGGCCCGTA
ACCGCCAGGCCCTGGAGATGTTTCTTGCCCCGCTCGCCATCCTCCCGTTTGACGAGGCGGCCGTGTGGGCCTATGGCGAC
CTGCGTGCCGATCTGGAGCGTCGAGGCAGCCCCATAGGCGCGCTTGACACCATGATCGCTGCCCACGCCCTCAGTCAGCG
GGCCCTGCTGGTCACCAACAACACCCGCGAATTCGCCAAGGTGCCCGGCCTGCAGTTGGACAACTGGGCTGTCGCTACCT
GA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT140154 WP_003156188.1 NZ_CP044132:527669-527950 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT140154 NZ_CP058970:c5472783-5472550 [Delftia acidovorans]
ATGGACACCGCACGCCTTTTCCAATCCGGCCGCAGTCAAGCCGTGCGGCTGCCCAAGGAATACCGTTTCACGGGCACTGA
AGTCGTGGTGAAGCACTTCGGCAACGGCGTGTTGCTGCTGCCCGCAGACGACCCTTGGCAAACACTGCAAGCCGGGCTGG
CTGAATTTGAACCGGGCTTTGTGCTTGAGCGCGAGCAGCCTGAGGCCCAGCAGCGGGACGAGATCAGCCCATGA
ATGGACACCGCACGCCTTTTCCAATCCGGCCGCAGTCAAGCCGTGCGGCTGCCCAAGGAATACCGTTTCACGGGCACTGA
AGTCGTGGTGAAGCACTTCGGCAACGGCGTGTTGCTGCTGCCCGCAGACGACCCTTGGCAAACACTGCAAGCCGGGCTGG
CTGAATTTGAACCGGGCTTTGTGCTTGAGCGCGAGCAGCCTGAGGCCCAGCAGCGGGACGAGATCAGCCCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|