Detailed information of TA system TA140153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 635519..636303
• Replicon: plasmid pRPC2
• Accession: NZ_CP044127
• Organism: Rhizobium sp. BG4

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: F2982_RS30700
• Protein ID: WP_203431310.1
• Coordinates: 635519..636016 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: F2982_RS30705
• Protein ID: WP_203431311.1
• Coordinates: 636013..636303 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2982_RS30680	632264..633526	-	1263	WP_112716948.1	type II toxin-antitoxin system HipA family toxin	-
F2982_RS30685	633523..633750	-	228	WP_112717548.1	helix-turn-helix transcriptional regulator	-
F2982_RS30690	633896..634193	-	298	Protein_603	HNH endonuclease	-
F2982_RS30695	635157..635366	-	210	Protein_604	aldo/keto reductase	-
F2982_RS30700	635519..636016	-	498	WP_203431310.1	GNAT family N-acetyltransferase	Toxin
F2982_RS30705	636013..636303	-	291	WP_203431311.1	DUF1778 domain-containing protein	Antitoxin
F2982_RS30710	636873..637820	-	948	WP_199628404.1	ABC transporter permease	-
F2982_RS30715	637822..638799	-	978	WP_203431312.1	ABC transporter permease	-
F2982_RS30720	638801..640282	-	1482	WP_203431642.1	sugar ABC transporter ATP-binding protein	-
F2982_RS30725	640282..641295	-	1014	WP_130279691.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..790983	790983

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-87)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 17692.30 Da
• Isoelectric Point: 8.9582

>T140153 WP_203431310.1 NZ_CP044127:c636016-635519 [Rhizobium sp. BG4]
VTLTSPAPLAEHHELVGFDSGVSELDDWLRRRARVNQASGASRTFVVCDGNRVVAYYALASGAVRTPEVPGRFKRNMPDP
IPVAGLGRLAIDRTYQGRGIGRALVRDAGLRLLNAAEILGIRGVLVHAISDDARTFYEAVGFLPSPSDPLMLMIGLKDLS
NALSA

Nucleotide

• Length: 498 bp

>T140153 NZ_CP058970:c1483085-1482702 [Delftia acidovorans]
ATGTCCACCCAGCCGCGCAAACCGCTCAAATGGGTCGGCTCGGCCAAGCGGGATCTGGACGGAATGCCCGAGGACGTCCA
AGACGTGTTCGGCCATGCGATCGACCTGGCGCAAGCCGGTGGCAAGCACCCGGACGCCAAGGCGCTGAGCGGGTTTGGTT
CCGCCGCGGTTTTGGAAGTGGTCGAGGATTTCCGGAGCGACACGTTCCGGGCGGTCTACACGGTCAAGTTCGCGGGCTGG
GTCTATGTGCTGCACTGTTTTCAGAAGAAGTCCAAGAGCGGCATCAAGACGCCGAAGGAGGACCTGGACCTGATCAAGGC
ACGGCTGAAGGCTGCGGTGCAGGATTTTGAGGCTTGGCAAGCAAAGCAAGGAGTAAAGCGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10981.70 Da
• Isoelectric Point: 10.0876

>AT140153 WP_203431311.1 NZ_CP044127:c636303-636013 [Rhizobium sp. BG4]
MPTKRKNLEASVPLNIRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTVFTVSPEVYAEYLTRLDAPAKPN
ARLKRTMLTQAPWDEA

Nucleotide

• Length: 291 bp

>AT140153 NZ_CP058970:c1482705-1482376 [Delftia acidovorans]
ATGAGCCAGAACAGCCACATCACCATCGAAGAAGGCAGCACCAACGTCTACGCGGACCTGGGCTATGCCGACGCTGCGGA
AATGCAGCGCAAGTCGCAGCTCGCGGCTGAGATTGCCCGCGCCATCAAGGCGCGACGCCTGACGCAACAGGGAGCCGCCG
CGCTGCTGGAAATCGATCAGTCCAAGATCTCCCGCATCACGCGCGGCCAGTTTCGCGGGGTCAGCGAGGCGAAGCTGCTG
GAACTGGTGGCCAAGCTGGGGCATGACGTGAAGATCGTGGTCGGTCCAGTACGGCGGCGTGCCGGTAAGATCGAGCTGCA
GTTCGCCTGA