Detailed information of TA system TA140152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 632264..633750
• Replicon: plasmid pRPC2
• Accession: NZ_CP044127
• Organism: Rhizobium sp. BG4

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: F2982_RS30680
• Protein ID: WP_112716948.1
• Coordinates: 632264..633526 (-)
• Length: 421 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: F2982_RS30685
• Protein ID: WP_112717548.1
• Coordinates: 633523..633750 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2982_RS30655	628181..628797	-	617	Protein_596	zeta toxin family protein	-
F2982_RS30660	628808..628981	-	174	WP_203431307.1	hypothetical protein	-
F2982_RS30665	629177..629440	-	264	WP_203431308.1	hypothetical protein	-
F2982_RS30670	630044..630396	+	353	Protein_599	type II toxin-antitoxin system RelE/ParE family toxin	-
F2982_RS30675	630386..630709	+	324	WP_203431309.1	DNA-binding transcriptional regulator	-
F2982_RS30680	632264..633526	-	1263	WP_112716948.1	type II toxin-antitoxin system HipA family toxin	Toxin
F2982_RS30685	633523..633750	-	228	WP_112717548.1	helix-turn-helix transcriptional regulator	Antitoxin
F2982_RS30690	633896..634193	-	298	Protein_603	HNH endonuclease	-
F2982_RS30695	635157..635366	-	210	Protein_604	aldo/keto reductase	-
F2982_RS30700	635519..636016	-	498	WP_203431310.1	GNAT family N-acetyltransferase	-
F2982_RS30705	636013..636303	-	291	WP_203431311.1	DUF1778 domain-containing protein	-
F2982_RS30710	636873..637820	-	948	WP_199628404.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..790983	790983

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-377)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-63)

Sequences

Toxin

Protein

• Length: 421 a.a.
• Molecular weight: 45743.47 Da
• Isoelectric Point: 5.6703

>T140152 WP_112716948.1 NZ_CP044127:c633526-632264 [Rhizobium sp. BG4]
VTRVLDVYFRDTKAGVLGRLDDGLLIFAYDGAYLLDQRSRAISFSMPLQEEPFGDQVVRPFFSGLLPDEGARQRLAGALG
LSAGNAFGLLEVIGGECAGALSIYPTGTAPEPPGQSQAEILTSERLDEVLSRLRTRPLLGGDEGIRLSLAGAQDKLAVIV
DGDSIALARDGRPTTHILKPVIQALEGTVENEYFCMQLAARLKLPVPHVQIRLDGATAFLLVERYDRTKAADGIIERLHQ
EDFCQALSVPPELKYEAEGGPGTERSLGLIDNACARPVADRLQFIRMLIFHYLVGNADAHGKNYALLYGSDEPDLAPIYD
VVCTAVYPRLSKRLAMKIGGRDVPDTIQLKHWATLVPETKSSQRILVRDLAQMAERIVSEADALVAELAHEGIRHPILSA
IRKVISSRAGLLQRAAEHVS

Nucleotide

• Length: 1263 bp

>T140152 NZ_CP058970:856669-857094 [Delftia acidovorans]
ATGATCGTTGTCGATACCAACGTCATTTCGGAACTCTGGAGGCTTGCGCCCGACTCCAGAGTGCTTGCCTGGATGGATGC
CCAGGCCATTGAAACGCTGCATCTGTCTGCCGTCACCGTGGCAGAACTGCGCTACGGCATTGCAGCGATGCCTGAGGGAA
GGCGCCGCACTATCTACCAGGAGCGACTGGAGCATGAGGTTCTGCCGGCCTTCGCCGGCCGTGTCCTGGCCTTTGACCTG
GACGCGGCAAAGGCCTATGCCGATCTGATGGCGAGCGCGAAGACGCAGGGCAGGGCCATCGCCAAGGCCGATGGCTATAT
AGCGGCCACTGCCATGGCGCATGGGCTGGTGGTGGCAACGCGCGACACCAGTCCGTTCGAGGCCGGTGGGCTGCAGACCA
TCAATCCCTGGACGGCAGCGCCCTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8205.62 Da
• Isoelectric Point: 11.4487

>AT140152 WP_112717548.1 NZ_CP044127:c633750-633523 [Rhizobium sp. BG4]
MDKIKDTKTLGMLIRQERRQQGLTQEQLAGAIGVGVRFLRELEAGKESCQIGRAMQVLAGLGLLVSVGRRQGPRS

Nucleotide

• Length: 228 bp

>AT140152 NZ_CP058970:856415-856672 [Delftia acidovorans]
ATGGCCAATGTGACTGTGAGAAACCTGCCCGATGCCGTGCACCGGGCCTTGCGGGTACGCGCTGCCCACCATGGCCGCAG
TACGGAGGCGGAAATCCGCGACATTCTGGAAGCCGCCGTCCGGCCGGCAGAGCGTGTCAGGCTGGGCTCCATGCTGGCGT
CCATTGCCCGCGAGGCAGGAGGACTGACCGACTCCGAAGCCGAGGGCTTCAACCGCCTGCGCGACAGGGCGCCAGCCGAG
CCCATGGACTTTGAATGA