Detailed information of TA system TA140151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 923681..924563
• Replicon: plasmid pRPC1
• Accession: NZ_CP044126
• Organism: Rhizobium sp. BG4

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: F2982_RS24425
• Protein ID: WP_203430206.1
• Coordinates: 923681..924199 (-)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: F2982_RS24430
• Protein ID: WP_130280273.1
• Coordinates: 924192..924563 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2982_RS24395	918696..919367	+	672	WP_130280265.1	amino acid ABC transporter permease	-
F2982_RS24400	919367..920038	+	672	WP_203430204.1	amino acid ABC transporter permease	-
F2982_RS24405	920040..920774	+	735	WP_199625558.1	amino acid ABC transporter ATP-binding protein	-
F2982_RS24410	920784..921701	+	918	WP_203430205.1	N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase	-
F2982_RS24415	921724..922515	+	792	WP_199625560.1	transporter substrate-binding domain-containing protein	-
F2982_RS24420	922552..923553	-	1002	WP_199625561.1	LacI family transcriptional regulator	-
F2982_RS24425	923681..924199	-	519	WP_203430206.1	RES domain-containing protein	Toxin
F2982_RS24430	924192..924563	-	372	WP_130280273.1	DUF2384 domain-containing protein	Antitoxin
F2982_RS24435	924643..925425	-	783	WP_203430207.1	crotonase/enoyl-CoA hydratase family protein	-
F2982_RS24440	925422..926282	-	861	WP_203430208.1	hydroxymethylglutaryl-CoA lyase	-
F2982_RS24445	926279..928258	-	1980	WP_203430209.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..1609137	1609137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (11-165)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (22-60)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 18935.39 Da
• Isoelectric Point: 6.7153

>T140151 WP_203430206.1 NZ_CP044126:c924199-923681 [Rhizobium sp. BG4]
MPDRNSGDILKLWRAFVPRWAFAPLSGEGAARFGGRWNPVGASTIYAARELSTAWAEYNQGFVQHPAIIAQLELAGARLA
DTTASAVLESYGEAADIHRCEWRQELDAGRIPTTHRLRERLIADGFDGVIYPSFMSPGGTCVALWRWNTPGSPELRAIDP
EGRLPKTAASWL

Nucleotide

• Length: 519 bp

>T140151 NZ_CP058962:29972-30277 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACTTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 12832.79 Da
• Isoelectric Point: 10.3104

>AT140151 WP_130280273.1 NZ_CP044126:c924563-924192 [Rhizobium sp. BG4]
MAAIGLDIAAARFGDGQSAFLSARRVADQLGVTLAELAKLIGVARNTLTAKSSARKVDGALSNVVRILSMASEMAGDDNR
AVIWFKHQPIPGWGGKTAFDLVGEGKSGKVLAYLEAVRSGVYA

Nucleotide

• Length: 372 bp

>AT140151 NZ_CP058962:29752-29970 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA