Detailed information of TA system TA140150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 467111..467763
• Replicon: plasmid pRPC1
• Accession: NZ_CP044126
• Organism: Rhizobium sp. BG4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F2982_RS22265
• Protein ID: WP_112717684.1
• Coordinates: 467111..467509 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: F2982_RS22270
• Protein ID: WP_112717682.1
• Coordinates: 467509..467763 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2982_RS22250	462797..464800	+	2004	WP_203430929.1	CocE/NonD family hydrolase	-
F2982_RS22255	464804..465715	-	912	WP_203430930.1	alpha/beta hydrolase	-
F2982_RS22260	465822..467015	-	1194	WP_203430931.1	acyltransferase	-
F2982_RS22265	467111..467509	-	399	WP_112717684.1	type II toxin-antitoxin system VapC family toxin	Toxin
F2982_RS22270	467509..467763	-	255	WP_112717682.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
F2982_RS22275	467927..468949	+	1023	WP_203431152.1	LacI family transcriptional regulator	-
F2982_RS22280	468990..470228	+	1239	WP_112717678.1	sugar ABC transporter substrate-binding protein	-
F2982_RS22285	470312..471226	+	915	WP_203430932.1	sugar ABC transporter permease	-
F2982_RS22290	471226..472089	+	864	WP_203430933.1	carbohydrate ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..1609137	1609137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14275.18 Da
• Isoelectric Point: 4.7324

>T140150 WP_112717684.1 NZ_CP044126:c467509-467111 [Rhizobium sp. BG4]
MVIDTSAILAIALNEPEAAAFEQRIADDPVRLISAATLLEAAMVLETRLGEAGGSELDLWIFKAGVEVVAVDADHADQAR
RAWRRYGKGRHAAGLNYGDCFSYALAMISGEPLLYKGNDFSQTDIRAAHAIR

Nucleotide

• Length: 399 bp

>T140150 NZ_CP058961:25582-25887 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACTTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9478.69 Da
• Isoelectric Point: 6.3871

>AT140150 WP_112717682.1 NZ_CP044126:c467763-467509 [Rhizobium sp. BG4]
MALSIKDPETERLARALAEKTGETITVATRKALEERLRRAGNDLRKAALLEDLAASRRRWRDMPVNDDRSADDILGYDET
GVPR

Nucleotide

• Length: 255 bp

>AT140150 NZ_CP058961:25362-25580 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA