Detailed information of TA system TA140149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3933424..3933965
• Replicon: chromosome
• Accession: NZ_CP044125
• Organism: Rhizobium sp. BG4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: F2982_RS19745
• Protein ID: WP_203428863.1
• Coordinates: 3933642..3933965 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: F2982_RS19740
• Protein ID: WP_203428862.1
• Coordinates: 3933424..3933645 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2982_RS19720	3929161..3930045	+	885	WP_203428861.1	alpha/beta hydrolase	-
F2982_RS19725	3930181..3930951	+	771	WP_112716271.1	triose-phosphate isomerase	-
F2982_RS19730	3931045..3931590	+	546	WP_112716269.1	preprotein translocase subunit SecG	-
F2982_RS19735	3931732..3933360	+	1629	WP_112716267.1	CTP synthase	-
F2982_RS19740	3933424..3933645	+	222	WP_203428862.1	antitoxin MazE family protein	Antitoxin
F2982_RS19745	3933642..3933965	+	324	WP_203428863.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F2982_RS19750	3934002..3934472	+	471	WP_203428864.1	SRPBCC family protein	-
F2982_RS19755	3934694..3936532	+	1839	WP_203428865.1	ABC transporter ATP-binding protein/permease	-
F2982_RS19760	3936568..3937656	-	1089	WP_203428866.1	citrate synthase/methylcitrate synthase	-
F2982_RS19765	3937741..3938877	+	1137	WP_203428867.1	citrate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (7-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11600.48 Da
• Isoelectric Point: 7.0128

>T140149 WP_203428863.1 NZ_CP044125:3933642-3933965 [Rhizobium sp. BG4]
MRRGDLVTVALSGDFGKPRPALIIQSDNFDATDTVTLLLISGTLVDAPLLRLTIDPDAENGLRKPSQIMIDKAMTVRRDR
IGQHVGRVSDEAMISVTRSLALFLGIA

Nucleotide

• Length: 324 bp

>T140149 NZ_CP058961:c24548-24132 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8077.08 Da
• Isoelectric Point: 5.8189

>AT140149 WP_203428862.1 NZ_CP044125:3933424-3933645 [Rhizobium sp. BG4]
MSSNVSARVKKRRDNLRAAGLRPVQIWVPDTRRPGFLDECRRQSAVVAKADADDAGLHGFMEEALADLDPAEE

Nucleotide

• Length: 222 bp

>AT140149 NZ_CP058961:c24775-24545 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA