Detailed information of TA system TA140146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 935719..936374
• Replicon: chromosome
• Accession: NZ_CP044125
• Organism: Rhizobium sp. BG4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F2982_RS04975
• Protein ID: WP_112712795.1
• Coordinates: 935955..936374 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: F2982_RS04970
• Protein ID: WP_203429440.1
• Coordinates: 935719..935958 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2982_RS04960	931176..934637	-	3462	WP_203429439.1	chromosome segregation protein SMC	-
F2982_RS04965	934815..935507	-	693	WP_199626362.1	DsbA family protein	-
F2982_RS04970	935719..935958	+	240	WP_203429440.1	Arc family DNA-binding protein	Antitoxin
F2982_RS04975	935955..936374	+	420	WP_112712795.1	type II toxin-antitoxin system VapC family toxin	Toxin
F2982_RS04980	936379..936864	-	486	WP_112714324.1	DUF721 domain-containing protein	-
F2982_RS04985	936960..938051	+	1092	WP_203430012.1	A/G-specific adenine glycosylase	-
F2982_RS04990	938055..938663	+	609	WP_203429441.1	HAD family phosphatase	-
F2982_RS04995	938666..939031	+	366	WP_203429442.1	nuclear transport factor 2 family protein	-
F2982_RS05000	939287..940207	+	921	WP_203429443.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (6-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15163.51 Da
• Isoelectric Point: 4.6190

>T140146 WP_112712795.1 NZ_CP044125:935955-936374 [Rhizobium sp. BG4]
MIILDTNVISEPMKPDADPKVLAWLDDQAAETLYLTSVNLAELLSGIEVLPDGRRKAGLHQKLVELMARLFEDRILPFDE
PAAREFAPLIGRARSAGRVISMADGQIAAIARTRGFTVATRDVAPFEAAGVPIINPWES

Nucleotide

• Length: 420 bp

>T140146 NZ_CP058960:c4921927-4921643 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8634.73 Da
• Isoelectric Point: 5.1425

>AT140146 WP_203429440.1 NZ_CP044125:935719-935958 [Rhizobium sp. BG4]
MSAIVVRNLPGETHRALKQRAAKNGRSTEAEIRSILEEAVRPSGRLKIGSELAAFAKEIGDADLIFERDQTPVDAADFE

Nucleotide

• Length: 240 bp

>AT140146 NZ_CP058960:c4922159-4921917 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA