Detailed information of TA system TA140144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 641308..641885
• Replicon: chromosome
• Accession: NZ_CP044125
• Organism: Rhizobium sp. BG4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: F2982_RS03445
• Protein ID: WP_203429234.1
• Coordinates: 641511..641885 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: F2982_RS03440
• Protein ID: WP_199626663.1
• Coordinates: 641308..641514 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2982_RS03415	636903..637877	-	975	WP_203429232.1	iron ABC transporter permease	-
F2982_RS03420	637925..638854	-	930	WP_203429233.1	ABC transporter substrate-binding protein	-
F2982_RS03425	638956..639429	-	474	WP_112719631.1	Lrp/AsnC family transcriptional regulator	-
F2982_RS03430	639572..640477	+	906	WP_199626665.1	DMT family transporter	-
F2982_RS03435	640527..641141	-	615	WP_112719633.1	L,D-transpeptidase	-
F2982_RS03440	641308..641514	+	207	WP_199626663.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
F2982_RS03445	641511..641885	+	375	WP_203429234.1	PIN domain-containing protein	Toxin
F2982_RS03450	641892..643556	-	1665	WP_203429235.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
F2982_RS03455	643813..644601	+	789	WP_203429236.1	SDR family oxidoreductase	-
F2982_RS03460	644791..646131	+	1341	WP_203429237.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13996.26 Da
• Isoelectric Point: 6.7715

>T140144 WP_203429234.1 NZ_CP044125:641511-641885 [Rhizobium sp. BG4]
MILIDSSIWIDHFRAADDYLDALLRSENVIIHPFVIGELSLGHISRYDAIMTMLADLPVISVADDAEVRHMIRSNKLFGT
GIGYIDAHLLASALLSQRHTLWTRDKRLHRIASTLGVAMPFHLH

Nucleotide

• Length: 375 bp

>T140144 NZ_CP058960:4207277-4207495 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7627.71 Da
• Isoelectric Point: 5.1106

>AT140144 WP_199626663.1 NZ_CP044125:641308-641514 [Rhizobium sp. BG4]
MRTTITLDDELLEKARRYTGVAETPTLIRLALEGLVQREAARRLADLGGTEPNIEAPPRRRFGPVEPE

Nucleotide

• Length: 207 bp

>AT140144 NZ_CP058960:4206876-4207250 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG