Detailed information of TA system TA140140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-PrlF
• Location: 237049..237592
• Replicon: chromosome
• Accession: NZ_CP044125
• Organism: Rhizobium sp. BG4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: F2982_RS01275
• Protein ID: WP_203429025.1
• Coordinates: 237049..237387 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: F2982_RS01280
• Protein ID: WP_112711913.1
• Coordinates: 237374..237592 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F2982_RS01250	233283..234686	-	1404	WP_203429023.1	DegQ family serine endoprotease	-
F2982_RS01255	234759..234896	+	138	WP_162708600.1	hypothetical protein	-
F2982_RS01260	234893..236143	-	1251	WP_203429024.1	sensor domain-containing diguanylate cyclase	-
F2982_RS01265	236298..236669	+	372	WP_112711493.1	DUF1232 domain-containing protein	-
F2982_RS01270	236701..237045	+	345	WP_112711492.1	hypothetical protein	-
F2982_RS01275	237049..237387	-	339	WP_203429025.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
F2982_RS01280	237374..237592	-	219	WP_112711913.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
F2982_RS01285	237741..239576	-	1836	WP_199629445.1	dihydroxy-acid dehydratase	-
F2982_RS01290	239925..240848	+	924	WP_203429996.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
F2982_RS01295	240848..241507	+	660	WP_203429026.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-
F2982_RS01300	241504..241911	-	408	WP_203429027.1	DUF2809 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-109)

Antitoxin

• PrlF antitoxin (4-57)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 11971.68 Da
• Isoelectric Point: 5.7974

>T140140 WP_203429025.1 NZ_CP044125:c237387-237049 [Rhizobium sp. BG4]
MPTFEQGDIIRVPFPYTDRDTRQRRPALVVSAGSLGENDALVWAVMITSAENRPWLGDVAISDHQTAGLPAPSVVRPAKI
ATVETSQIDRIGRLPDAALAVVISAIRAQLGG

Nucleotide

• Length: 339 bp

>T140140 NZ_CP058960:736778-737263 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7978.91 Da
• Isoelectric Point: 4.4865

>AT140140 WP_112711913.1 NZ_CP044125:c237592-237374 [Rhizobium sp. BG4]
MITSKMTSKAQTTIPQAVRSALHLKEGDSIVYTLEADNRVVISRAAEPMSDDPFATFSEWDSEADRKAYADL

Nucleotide

• Length: 219 bp

>AT140140 NZ_CP058960:736488-736781 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA