Detailed information of TA system TA140122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1523762..1525365
• Replicon: chromosome
• Accession: NZ_CP044121
• Organism: Raoultella planticola strain S25

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: F3X94_RS07495
• Protein ID: WP_134935334.1
• Coordinates: 1524034..1525365 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2X2EM10
• Locus tag: F3X94_RS07490
• Protein ID: WP_032696205.1
• Coordinates: 1523762..1524034 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3X94_RS07465	1519389..1520630	+	1242	WP_032699861.1	sensor domain-containing diguanylate cyclase	-
F3X94_RS07470	1520649..1521281	+	633	WP_099973191.1	class I SAM-dependent methyltransferase	-
F3X94_RS07475	1521497..1522450	+	954	WP_032687742.1	hypothetical protein	-
F3X94_RS07480	1522583..1523008	+	426	WP_032687741.1	hypothetical protein	-
F3X94_RS07485	1523014..1523568	+	555	WP_046852510.1	RNA polymerase sigma factor	-
F3X94_RS07490	1523762..1524034	+	273	WP_032696205.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
F3X94_RS07495	1524034..1525365	+	1332	WP_134935334.1	type II toxin-antitoxin system HipA family toxin	Toxin
F3X94_RS07500	1525298..1526704	-	1407	WP_134935744.1	glycoside hydrolase family 10 protein	-
F3X94_RS07505	1527417..1528475	+	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
F3X94_RS07510	1528575..1529489	+	915	WP_015584333.1	branched-chain amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49529.83 Da
• Isoelectric Point: 6.5069

>T140122 WP_134935334.1 NZ_CP044121:1524034-1525365 [Raoultella planticola]
MATLVTWMNNERVGELIKQASGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDDAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENATRRALSWEALDSHALDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGEQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALDFAVPRASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLRLSMSLKASKGRKTEIQTLYPRHFLSTAKEVGFAEEQMLEILRYFVDNVP
HAVAAVKETLPHDFSPPVYEAITDRLLWVHSRLQNAMAALPSG

Nucleotide

• Length: 1332 bp

>T140122 NZ_CP058948:c3670607-3670293 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10168.43 Da
• Isoelectric Point: 4.9491

>AT140122 WP_032696205.1 NZ_CP044121:1523762-1524034 [Raoultella planticola]
MSPSPGIYSPVQLANYLKLVRQKNQWTQSDLAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTND
SSDNPQDLDW

Nucleotide

• Length: 273 bp

>AT140122 NZ_CP058948:c3670296-3669880 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X2EM10