Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 30746..31365 Replicon chromosome
Accession NZ_CP044121
Organism Raoultella planticola strain S25

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag F3X94_RS00625 Protein ID WP_004858783.1
Coordinates 30746..30964 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag F3X94_RS00630 Protein ID WP_004858785.1
Coordinates 30991..31365 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3X94_RS00595 26820..27080 + 261 WP_032689208.1 type B 50S ribosomal protein L31 -
F3X94_RS00600 27083..27223 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
F3X94_RS00605 27220..27930 - 711 WP_150340985.1 GNAT family N-acetyltransferase -
F3X94_RS00610 28032..29483 + 1452 WP_150340986.1 PLP-dependent aminotransferase family protein -
F3X94_RS00615 29458..29928 - 471 WP_004858780.1 YlaC family protein -
F3X94_RS00620 30028..30594 - 567 WP_032689205.1 maltose O-acetyltransferase -
F3X94_RS00625 30746..30964 - 219 WP_004858783.1 hemolysin expression modulator Hha Toxin
F3X94_RS00630 30991..31365 - 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
F3X94_RS00635 31892..35038 - 3147 WP_032689204.1 multidrug efflux RND transporter permease subunit -
F3X94_RS00640 35061..36254 - 1194 WP_032689203.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T140121 WP_004858783.1 NZ_CP044121:c30964-30746 [Raoultella planticola]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T140121 NZ_CP058948:c3604696-3604400 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT140121 WP_004858785.1 NZ_CP044121:c31365-30991 [Raoultella planticola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT140121 NZ_CP058948:c3604398-3604003 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References