Detailed information of TA system TA140120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 43517..44148
• Replicon: chromosome
• Accession: NZ_CP044115
• Organism: Roseomonas mucosa strain FDAARGOS_658

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOB66_RS01570
• Protein ID: WP_019463221.1
• Coordinates: 43738..44148 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1S8D2B6
• Locus tag: FOB66_RS01565
• Protein ID: WP_019463222.1
• Coordinates: 43517..43741 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB66_RS01540	39294..40570	+	1277	WP_085977433.1	IS3 family transposase	-
FOB66_RS01545	40587..41360	-	774	WP_026033587.1	hypothetical protein	-
FOB66_RS01550	41643..41879	+	237	WP_019463225.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FOB66_RS01555	41876..42283	+	408	WP_019463224.1	PIN domain-containing protein	-
FOB66_RS01560	42280..43404	+	1125	WP_019463223.1	site-specific integrase	-
FOB66_RS01565	43517..43741	+	225	WP_019463222.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FOB66_RS01570	43738..44148	+	411	WP_019463221.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOB66_RS01575	44206..46881	+	2676	WP_167522604.1	CRISPR-associated helicase Cas3'	-
FOB66_RS01580	46958..48574	+	1617	WP_026033586.1	type I-E CRISPR-associated protein Cse1/CasA	-
FOB66_RS01585	48574..49131	+	558	WP_171982328.1	type I-E CRISPR-associated protein Cse2/CasB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

• SpoVT-AbrB domain (9-45)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15007.19 Da
• Isoelectric Point: 6.9320

>T140120 WP_019463221.1 NZ_CP044115:43738-44148 [Roseomonas mucosa]
VTLRYLLDTNLCIRVLRDRPAGLRGRFNAEAEALCTSDVVLYELLYGAERSARPAEMRREVERFVARLSVLPFDGDAAAH
TAEIRASLERRGAVIGPYDLMIAGQARSRGLVVVTGNLSEFGRVEGLRCEDWLAGA

Nucleotide

• Length: 411 bp

>T140120 NZ_CP058948:c3476975-3476568 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8191.34 Da
• Isoelectric Point: 4.9987

>AT140120 WP_019463222.1 NZ_CP044115:43517-43741 [Roseomonas mucosa]
MPARTTLFLSNRSQAVRLPKGVAFGEGVREVVILRDGPRRVIAPADALWDDFFAGPGVDLDDRQQPAMQEREAL

Nucleotide

• Length: 225 bp

>AT140120 NZ_CP058948:c3477222-3476956 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1S8D2B6