Detailed information of TA system TA140115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3493113..3493852
• Replicon: chromosome
• Accession: NZ_CP044107
• Organism: Enterobacter hormaechei strain FDAARGOS_642

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FOB50_RS16560
• Protein ID: WP_045329830.1
• Coordinates: 3493367..3493852 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: FOB50_RS16555
• Protein ID: WP_003857131.1
• Coordinates: 3493113..3493379 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB50_RS16535	3488589..3489575	+	987	WP_017382341.1	DUF979 domain-containing protein	-
FOB50_RS16540	3489585..3490580	+	996	WP_048973857.1	DUF2891 domain-containing protein	-
FOB50_RS16545	3490602..3491990	-	1389	WP_150391135.1	MFS transporter	-
FOB50_RS16550	3492120..3493049	+	930	WP_048973859.1	LysR family transcriptional regulator	-
FOB50_RS16555	3493113..3493379	+	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
FOB50_RS16560	3493367..3493852	+	486	WP_045329830.1	GNAT family N-acetyltransferase	Toxin
FOB50_RS16565	3494033..3494500	+	468	Protein_3196	hypothetical protein	-
FOB50_RS16570	3494778..3495224	+	447	WP_014069654.1	DUF3290 domain-containing protein	-
FOB50_RS16575	3495234..3495869	+	636	WP_015570517.1	DUF421 domain-containing protein	-
FOB50_RS16580	3496053..3496598	-	546	WP_185855161.1	NUDIX hydrolase	-
FOB50_RS16585	3496667..3497959	-	1293	WP_048973861.1	glycoside hydrolase	-
FOB50_RS16590	3498064..3498822	-	759	WP_045341969.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17512.18 Da
• Isoelectric Point: 9.9658

>T140115 WP_045329830.1 NZ_CP044107:3493367-3493852 [Enterobacter hormaechei]
VGRVTAPEPLSSAHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T140115 NZ_CP058948:c2521725-2521471 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT140115 WP_003857131.1 NZ_CP044107:3493113-3493379 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT140115 NZ_CP058948:c2521973-2521722 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9