Detailed information of TA system TA140105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1196094..1196710
• Replicon: chromosome
• Accession: NZ_CP044107
• Organism: Enterobacter hormaechei strain FDAARGOS_642

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOB50_RS05730
• Protein ID: WP_150390848.1
• Coordinates: 1196094..1196465 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: FOB50_RS05735
• Protein ID: WP_015569912.1
• Coordinates: 1196468..1196710 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB50_RS05715	1193594..1194496	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
FOB50_RS05720	1194493..1195128	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
FOB50_RS05725	1195125..1196054	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
FOB50_RS05730	1196094..1196465	-	372	WP_150390848.1	PIN domain-containing protein	Toxin
FOB50_RS05735	1196468..1196710	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
FOB50_RS05740	1196909..1197901	+	993	WP_058700982.1	alpha/beta hydrolase	-
FOB50_RS05745	1197838..1198779	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
FOB50_RS05750	1198824..1199261	-	438	WP_032668943.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FOB50_RS05755	1199258..1200139	-	882	WP_048973924.1	virulence factor BrkB family protein	-
FOB50_RS05760	1200133..1200732	-	600	WP_017694048.1	glucose-1-phosphatase	-
FOB50_RS05765	1200851..1201651	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13707.85 Da
• Isoelectric Point: 6.7561

>T140105 WP_150390848.1 NZ_CP044107:c1196465-1196094 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITRMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T140105 NZ_CP058948:c863476-863258 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT140105 WP_015569912.1 NZ_CP044107:c1196710-1196468 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT140105 NZ_CP058948:c863876-863502 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3