Detailed information of TA system TA140093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 174931..175460
• Replicon: chromosome
• Accession: NZ_CP044106
• Organism: Staphylococcus aureus strain FDAARGOS_660

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FOB68_RS01085
• Protein ID: WP_000621175.1
• Coordinates: 175098..175460 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FOB68_RS01080
• Protein ID: WP_000948331.1
• Coordinates: 174931..175101 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB68_RS01050	169967..170527	+	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-
FOB68_RS01055	170736..171215	+	480	WP_001287083.1	hypothetical protein	-
FOB68_RS01060	171208..172791	+	1584	WP_001294642.1	PH domain-containing protein	-
FOB68_RS01065	172778..173269	+	492	WP_001205915.1	PH domain-containing protein	-
FOB68_RS01070	173273..173632	+	360	WP_000581194.1	holo-ACP synthase	-
FOB68_RS01075	173698..174846	+	1149	WP_001280702.1	alanine racemase	-
FOB68_RS01080	174931..175101	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FOB68_RS01085	175098..175460	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FOB68_RS01090	175809..176810	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FOB68_RS01095	176929..177255	+	327	WP_001052491.1	anti-sigma factor antagonist	-
FOB68_RS01100	177257..177736	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
FOB68_RS01105	177711..178481	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T140093 WP_000621175.1 NZ_CP044106:175098-175460 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T140093 NZ_CP058940:2212036-2212404 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGTATTACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT140093 WP_000948331.1 NZ_CP044106:174931-175101 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT140093 NZ_CP058940:2211818-2212039 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCTGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1