Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1520145..1520765 Replicon chromosome
Accession NZ_CP044101
Organism Citrobacter werkmanii strain FDAARGOS_616

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FOB24_RS07845 Protein ID WP_002892050.1
Coordinates 1520145..1520363 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag FOB24_RS07850 Protein ID WP_003021733.1
Coordinates 1520391..1520765 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB24_RS07810 1515504..1516133 + 630 WP_150344739.1 hypothetical protein -
FOB24_RS07815 1516198..1516551 + 354 WP_042310059.1 DUF1428 family protein -
FOB24_RS07820 1516603..1518156 - 1554 WP_085047627.1 EAL domain-containing protein -
FOB24_RS07825 1518345..1518605 + 261 WP_038638331.1 type B 50S ribosomal protein L31 -
FOB24_RS07830 1518607..1518747 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
FOB24_RS07835 1518821..1519291 - 471 WP_150344740.1 YlaC family protein -
FOB24_RS07840 1519408..1519959 - 552 WP_150344741.1 maltose O-acetyltransferase -
FOB24_RS07845 1520145..1520363 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
FOB24_RS07850 1520391..1520765 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
FOB24_RS07855 1521253..1524402 - 3150 WP_038638345.1 multidrug efflux RND transporter permease subunit -
FOB24_RS07860 1524425..1525618 - 1194 WP_038638348.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T140073 WP_002892050.1 NZ_CP044101:c1520363-1520145 [Citrobacter werkmanii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T140073 NZ_CP058886:c3931780-3931493 [Shigella flexneri]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT140073 WP_003021733.1 NZ_CP044101:c1520765-1520391 [Citrobacter werkmanii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT140073 NZ_CP058886:c3931506-3931204 [Shigella flexneri]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References