Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 809551..811101 Replicon chromosome
Accession NZ_CP044101
Organism Citrobacter werkmanii strain FDAARGOS_616

Toxin (Protein)


Gene name hipA Uniprot ID A0A5P2M5J7
Locus tag FOB24_RS04550 Protein ID WP_064188549.1
Coordinates 809806..811101 (+) Length 432 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5P2M8A1
Locus tag FOB24_RS04545 Protein ID WP_032724128.1
Coordinates 809551..809802 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOB24_RS04530 805817..806409 - 593 Protein_725 tyrosine-type recombinase/integrase -
FOB24_RS04535 806610..807761 - 1152 WP_150344560.1 hypothetical protein -
FOB24_RS04540 807904..809013 + 1110 WP_064188548.1 hypothetical protein -
FOB24_RS04545 809551..809802 + 252 WP_032724128.1 helix-turn-helix domain-containing protein Antitoxin
FOB24_RS04550 809806..811101 + 1296 WP_064188549.1 type II toxin-antitoxin system HipA family toxin Toxin
FOB24_RS04555 811189..811455 + 267 WP_064188550.1 hypothetical protein -
FOB24_RS04560 811567..812220 + 654 WP_150344561.1 HNH endonuclease -
FOB24_RS04565 812276..813865 - 1590 WP_150344562.1 TraI domain-containing protein -
FOB24_RS04570 814087..814467 + 381 WP_150344563.1 RidA family protein -
FOB24_RS04575 814507..814878 - 372 WP_150344564.1 hypothetical protein -
FOB24_RS04580 814943..815848 - 906 WP_150344565.1 DUF1738 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 799828..874999 75171


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-398)

Antitoxin

Cro/C1-type helix-turn-helix domain

(10-64)


Sequences

Toxin        


Download         Length: 432 a.a.        Molecular weight: 49221.62 Da        Isoelectric Point: 7.2369

>T140072 WP_064188549.1 NZ_CP044101:809806-811101 [Citrobacter werkmanii]
VERLTVALNGIIVGTLEKTAGGEMSFYYQRDWLERAGSRAISLSMPLREERYRGEIVYNFFDNLLPDSEQIRSRIQARFH
APTKQPFDLLARIGMDCVGAIQLWPYETDIPSIQRVDAEPLTENEIEHLLQGYKDAPLGMQAEVDDFRISLAGAQEKTAL
LWWNNRWCRPRGSTPTSHIFKLPIGYIANNGIDLRESVENEWLCSKIAAEFGFPVADTRVAYFGQQKILIVERFDRRWAK
DGQWLMRLPTEDFCQALGVAPALKYESDGGPGIARSMDLLLGSQQPLKDRILFFRSQIFFWMLAAIDGHAKNFSVFLEPN
SAYRMAPLYDVMSAYPLMHPKGIPPRKAKMAMALSGRNRHFHWSTIMPRHFLSTAREVSFPVLQVEQLLAEMKQRTESVI
SKVETMLPAGFPEHISEAIFEGLRHQSARIP

Download         Length: 1296 bp

>T140072 NZ_CP058886:3618037-3618315 [Shigella flexneri]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9506.81 Da        Isoelectric Point: 9.9484

>AT140072 WP_032724128.1 NZ_CP044101:809551-809802 [Citrobacter werkmanii]
MNVTSPKMLAQAVRDARKQRKLTQREAAESMSMKQSTVSEFENHPEGTRLDTLFKLLAALDLELQVVSRKGKNKTSEGWD
QEW

Download         Length: 252 bp

>AT140072 NZ_CP058886:3617774-3618034 [Shigella flexneri]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5P2M5J7

Antitoxin

Source ID Structure
AlphaFold DB A0A5P2M8A1

References