Detailed information of TA system TA140069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4913723..4914459
• Replicon: chromosome
• Accession: NZ_CP044098
• Organism: Citrobacter portucalensis strain FDAARGOS_617

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FOB25_RS23490
• Protein ID: WP_032943446.1
• Coordinates: 4913977..4914459 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: FOB25_RS23485
• Protein ID: WP_008784769.1
• Coordinates: 4913723..4913989 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB25_RS23465	4908784..4909971	+	1188	WP_032943450.1	outer membrane protein transport protein	-
FOB25_RS23470	4910211..4910840	+	630	WP_008784772.1	winged helix-turn-helix transcriptional regulator	-
FOB25_RS23475	4910957..4911343	-	387	WP_008784771.1	hypothetical protein	-
FOB25_RS23480	4911587..4913569	+	1983	WP_032943448.1	MBL fold metallo-hydrolase	-
FOB25_RS23485	4913723..4913989	+	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
FOB25_RS23490	4913977..4914459	+	483	WP_032943446.1	GNAT family N-acetyltransferase	Toxin
FOB25_RS23495	4914756..4915943	-	1188	WP_032943443.1	outer membrane protein transport protein	-
FOB25_RS23500	4916036..4917151	-	1116	WP_032943441.1	DUF4056 domain-containing protein	-
FOB25_RS23505	4917152..4918282	-	1131	WP_032943439.1	BamA/TamA family outer membrane protein	-
FOB25_RS23510	4918282..4919220	-	939	WP_032943437.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4910957..4924144	13187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17657.46 Da
• Isoelectric Point: 9.5814

>T140069 WP_032943446.1 NZ_CP044098:4913977-4914459 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQSLGAARTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHVLTDEAKRFYLHHGFKASQLQERTLFLRLS

Nucleotide

• Length: 483 bp

>T140069 NZ_CP058886:2594659-2594766 [Shigella flexneri]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT140069 WP_008784769.1 NZ_CP044098:4913723-4913989 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT140069 NZ_CP058886:c2594612-2594546 [Shigella flexneri]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT