Detailed information of TA system TA140059

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3293714..3294368
• Replicon: chromosome
• Accession: NZ_CP044098
• Organism: Citrobacter portucalensis strain FDAARGOS_617

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: FOB25_RS15780
• Protein ID: WP_003825515.1
• Coordinates: 3293961..3294368 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: FOB25_RS15775
• Protein ID: WP_003825514.1
• Coordinates: 3293714..3293980 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB25_RS15745	3288868..3290301	-	1434	WP_032944895.1	6-phospho-beta-glucosidase BglA	-
FOB25_RS15750	3290423..3291151	-	729	WP_008785744.1	MurR/RpiR family transcriptional regulator	-
FOB25_RS15755	3291204..3291515	+	312	WP_008785743.1	N(4)-acetylcytidine aminohydrolase	-
FOB25_RS15760	3291679..3292341	+	663	WP_006686898.1	hemolysin III family protein	-
FOB25_RS15765	3292473..3293456	-	984	WP_032944893.1	tRNA-modifying protein YgfZ	-
FOB25_RS15775	3293714..3293980	+	267	WP_003825514.1	FAD assembly factor SdhE	Antitoxin
FOB25_RS15780	3293961..3294368	+	408	WP_003825515.1	protein YgfX	Toxin
FOB25_RS15785	3294470..3294991	-	522	WP_003026933.1	flavodoxin FldB	-
FOB25_RS15790	3295105..3296001	+	897	WP_003825519.1	site-specific tyrosine recombinase XerD	-
FOB25_RS15795	3296025..3296738	+	714	WP_003825520.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
FOB25_RS15800	3296744..3298477	+	1734	WP_032944891.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15882.76 Da
• Isoelectric Point: 11.3523

>T140059 WP_003825515.1 NZ_CP044098:3293961-3294368 [Citrobacter portucalensis]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQG

Nucleotide

• Length: 408 bp

>T140059 NZ_CP058886:c724372-724076 [Shigella flexneri]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10615.14 Da
• Isoelectric Point: 5.1700

>AT140059 WP_003825514.1 NZ_CP044098:3293714-3293980 [Citrobacter portucalensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADEQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT140059 NZ_CP058886:c724074-723679 [Shigella flexneri]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA