Detailed information of TA system TA140058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2699438..2700024
• Replicon: chromosome
• Accession: NZ_CP044098
• Organism: Citrobacter portucalensis strain FDAARGOS_617

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: FOB25_RS12770
• Protein ID: WP_032945247.1
• Coordinates: 2699656..2700024 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: FOB25_RS12765
• Protein ID: WP_032945249.1
• Coordinates: 2699438..2699659 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB25_RS12735	2694949..2695875	+	927	WP_003827575.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
FOB25_RS12740	2695872..2697149	+	1278	WP_032945254.1	branched chain amino acid ABC transporter permease LivM	-
FOB25_RS12745	2697146..2697913	+	768	WP_003023438.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
FOB25_RS12750	2697931..2698644	+	714	WP_003827581.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
FOB25_RS12755	2698746..2699027	+	282	WP_032945252.1	hypothetical protein	-
FOB25_RS12760	2698996..2699379	+	384	WP_032945251.1	hypothetical protein	-
FOB25_RS12765	2699438..2699659	+	222	WP_032945249.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FOB25_RS12770	2699656..2700024	+	369	WP_032945247.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
FOB25_RS12775	2700282..2701598	+	1317	WP_003827583.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
FOB25_RS12780	2701708..2702595	+	888	WP_032945245.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
FOB25_RS12785	2702592..2703437	+	846	WP_003023450.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
FOB25_RS12790	2703440..2704510	+	1071	WP_032945244.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2693778..2705250	11472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (25-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13455.72 Da
• Isoelectric Point: 6.7249

>T140058 WP_032945247.1 NZ_CP044098:2699656-2700024 [Citrobacter portucalensis]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIAVPANPDFVAMTVEAAAGQLTLEQIASRLRG

Nucleotide

• Length: 369 bp

>T140058 NZ_CP058886:649806-650120 [Shigella flexneri]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8292.38 Da
• Isoelectric Point: 4.7906

>AT140058 WP_032945249.1 NZ_CP044098:2699438-2699659 [Citrobacter portucalensis]
MRTMNYSEARQNLAEVLESAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMVHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT140058 NZ_CP058886:650117-650533 [Shigella flexneri]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA