Detailed information of TA system TA140054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4366112..4366728
• Replicon: chromosome
• Accession: NZ_CP044097
• Organism: Citrobacter koseri strain FDAARGOS_646

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A8AL49
• Locus tag: FOB54_RS20755
• Protein ID: WP_012133919.1
• Coordinates: 4366354..4366728 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FOB54_RS20750
• Protein ID: WP_162790489.1
• Coordinates: 4366112..4366357 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB54_RS20730	4362991..4363590	+	600	WP_047459890.1	glucose-1-phosphatase	-
FOB54_RS20735	4363584..4364456	+	873	WP_012133924.1	virulence factor BrkB family protein	-
FOB54_RS20740	4364453..4364890	+	438	WP_047459887.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FOB54_RS20745	4364935..4365876	+	942	WP_024130670.1	fatty acid biosynthesis protein FabY	-
FOB54_RS20750	4366112..4366357	+	246	WP_162790489.1	ribbon-helix-helix domain-containing protein	Antitoxin
FOB54_RS20755	4366354..4366728	+	375	WP_012133919.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOB54_RS20760	4366765..4367694	-	930	WP_047459875.1	formate dehydrogenase accessory protein FdhE	-
FOB54_RS20765	4367691..4368326	-	636	WP_049011887.1	formate dehydrogenase cytochrome b556 subunit	-
FOB54_RS20770	4368323..4369225	-	903	WP_071259455.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13741.00 Da
• Isoelectric Point: 9.8061

>T140054 WP_012133919.1 NZ_CP044097:4366354-4366728 [Citrobacter koseri]
MIKGPVLFDTNILIDLFSGRGEAKKALEAYSPQNAISLITWMEVMVGAKKYHQESRTRIALSAFNVIGVSQDIAERSVSL
RQEYGMKLPDAVILATAHIHRFALVTRNTKDFAGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T140054 NZ_CP058881:c46802-46422 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATTCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACTTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGTGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTTCGGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9524.75 Da
• Isoelectric Point: 4.1961

>AT140054 WP_162790489.1 NZ_CP044097:4366112-4366357 [Citrobacter koseri]
MMAGMEMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISDALGLWQGCEEDGVEYERKLREEW
Q

Nucleotide

• Length: 246 bp

>AT140054 NZ_CP058881:c47023-46802 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A8AL49