Detailed information of TA system TA140044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1899979..1900618
• Replicon: chromosome
• Accession: NZ_CP044097
• Organism: Citrobacter koseri strain FDAARGOS_646

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A381H3Z1
• Locus tag: FOB54_RS08990
• Protein ID: WP_071818852.1
• Coordinates: 1900442..1900618 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FOB54_RS08985
• Protein ID: WP_123907537.1
• Coordinates: 1899979..1900395 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB54_RS08965	1896216..1896389	-	174	WP_049008504.1	GhoT/OrtT family toxin	-
FOB54_RS08970	1896581..1896676	+	96	WP_071818853.1	stress response membrane protein YncL	-
FOB54_RS08975	1896775..1898199	-	1425	WP_012132353.1	aminobutyraldehyde dehydrogenase	-
FOB54_RS08980	1898519..1899928	-	1410	WP_114847892.1	PLP-dependent aminotransferase family protein	-
FOB54_RS08985	1899979..1900395	-	417	WP_123907537.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
FOB54_RS08990	1900442..1900618	-	177	WP_071818852.1	type II toxin-antitoxin system HicA family toxin	Toxin
FOB54_RS08995	1900838..1902799	-	1962	WP_114847929.1	U32 family peptidase	-
FOB54_RS09000	1902878..1903414	-	537	WP_104868641.1	XRE family transcriptional regulator	-
FOB54_RS09005	1903507..1904673	+	1167	WP_104868640.1	benzoate/H(+) symporter BenE family transporter	-
FOB54_RS09010	1904650..1905525	-	876	WP_012132344.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-68)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6810.81 Da
• Isoelectric Point: 10.8122

>T140044 WP_071818852.1 NZ_CP044097:c1900618-1900442 [Citrobacter koseri]
VKQSEFRRWLESQGVDVTNGTNHLKLRYHDKRSVMPRHPGDEIKDTLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T140044 NZ_CP058879:3628620-3628838 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15371.57 Da
• Isoelectric Point: 4.4377

>AT140044 WP_123907537.1 NZ_CP044097:c1900395-1899979 [Citrobacter koseri]
MRYPVNLIPAEEGGYVVSFPDIPEALTQGDTRHESLEAAQGALVTAFEFYFEDNQPIPLPSMVDEADDYVEIPLSIASKV
LLLNAFLESKITQQELARRIGRPKQEITRLFDLKHATKIDAVQNAARALGKELSLTML

Nucleotide

• Length: 417 bp

>AT140044 NZ_CP058879:3628220-3628594 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A381H3Z1